Proto-oncogene fos is expressed during development, differentiation, and growth.
Proto-oncogene fos is expressed during development, differentiation, and growth.
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原癌基因fos在发育、分化和生长过程中表达。
DOI：
10.1101/sqb.1985.050.01.091
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Verma,IM
中科院分区：
文献类型：
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作者：
Deschamps,J;Mitchell,RL;Meijlink,F;Kruijer,W;Schubert,D;Verma,IM
原癌基因fos在小鼠出生前和出生后的发育、细胞分化和细胞增殖过程中表达。第17天至第18天的小鼠羊膜显示高水平的fos特异性2.2-kb转录物以及55 K los蛋白。骨髓细胞和巨噬细胞系表达fos转录本。当人前单核细胞系U937或单粒细胞系HL-60用佛波酯（TPA）处理以分化为成熟巨噬细胞时，见证了fos基因表达的快速诱导。最高水平的诱导发生在30分钟，但fos RNA的合成是连续的，尽管低三到四倍，持续96-108小时。而fos蛋白在诱导后90-120分钟才能被检测到。当加入神经生长因子（NGF）后，PCI 2细胞分化形成神经突时，fos基因被快速但短暂地诱导，20-30分钟时达到最大水平。与单核细胞分化不同，加入NGE Rapid 120-240分钟后，没有观察到fos特异性转录物，当静止的小鼠成纤维细胞用有丝分裂原刺激增殖时，也观察到fos基因表达的瞬时诱导。在试图理解fos基因转录的调控中，转录增强子元件被定位于5 '帽核苷酸上游-60到400个核苷酸之间。我们讨论了复杂的调控thefos基因在正常细胞中的作用，并在体外诱导细胞转化的能力。
Proto-oncogene fos is expressed during pre-and postnatal development of the mouse, cellular differentiation, and cell proliferation. Day-17 to-18 mouse amnion displays high levels of both fos-specific 2.2-kb transcripts as well as 55K los protein. Bone marrow cells and macrophage cell lines express fos transcripts. Rapid induction of fos gene expression is witnessed when either a human promonocytic cell line, U937, or monomyelocytic cell line, HL-60, is treated with phorbol ester (TPA) to differentiate to mature macrophages. The highest levels of induction occur by 30 minutes but fos RNA is synthesized continuously, albeit three-to fourfold lower, for 96-108 hours. In contrast, fos protein can be detected for only 90-120 minutes postinduction. When PCI2 cells differentiate to form neurites upon addition of nerve growth factor (NGF), thefos gene is rapidly but transiently induced, with maximal levels accumulating by 20-30 minutes. Unlike the monocytic cell differentiation, no fos-spe-cific transcripts could be observed after 120-240 minutes of addition of NGE Rapid and transient induction of the expression of thefos gene is also observed when quiescent mouse fibroblasts are stimulated to proliferate with mitogens. In an attempt to understand the regulation of transcription of the fos gene, a transcriptional enhancer element has been localized to reside between-60 to-400 nucleotides upstream of the 5'cap nucleotide. We discuss the complex regulation of thefos gene in terms of its role in normal cells and its ability to induce cellular transformation in vitro.