Flow-cytometric method for counting very low numbers of leukocytes in platelet products.
Flow-cytometric method for counting very low numbers of leukocytes in platelet products.
复制标题
用于计数血小板产品中极少量白细胞的流式细胞术方法。
DOI：
10.1111/j.1423-0410.1990.tb00850.x
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发表时间：
1990
期刊：
影响因子：
2.7
通讯作者：
Cusack,WF
中科院分区：
文献类型：
--
作者：
Dzik,WH;Ragosta,A;Cusack,WF
We provide a detailed description of a new method for counting very low numbers of leukocytes present in platelet products. The method uses fluorescent staining of leukocyte DNA using propidium iodide and flow cytometry. The assay is accurate over a range of leukocyte concentrations from 0.1 to 10 WBC/ul. We validated the method using specially designed dilution studies and applied it to the measurement of leukocyte content in leukocyte-depleted platelet products prepared by apheresis or filtration. This method should prove useful in studies which address the clinical impact of leukocyte-depleted platelet products.
