Beta-endorphin response to an acute pain stimulus.
Beta-endorphin response to an acute pain stimulus.
复制标题
β-内啡肽对急性疼痛刺激的反应。
DOI：
10.1016/j.jneumeth.2008.10.013
复制
发表时间：
2009
影响因子：
3
通讯作者：
Farr,LynneA
中科院分区：
文献类型：
--
作者：
Rasmussen,NatalieAnn;Farr,LynneA
生物样品（例如生物标志物）的测量时间并不总是标准化的。生物标志物是最近许多研究和治疗的焦点。本研究的目的是确定暴露于疼痛和/或处理压力后β-内啡肽（BE）（一种可能的生物标志物）的释放时间，以使测量标准化。在处理后收集小鼠血浆用于BE分析，即由研究者采集、暴露于疼痛（55°C热板）或暴露于非疼痛刺激（室温热板）。暴露于疼痛或非疼痛刺激的组释放BE以响应刺激，但暴露于疼痛刺激的小鼠的反应持续时间长于暴露于非疼痛刺激的小鼠。暴露于非疼痛刺激的小鼠的BE在1分钟时达到峰值，并在5分钟时恢复到基线水平，而暴露于疼痛刺激的小鼠的BE反应在10分钟时达到峰值，并保持升高25分钟。这项研究的结果表明，BE可以作为疼痛和处理压力的生物标志物，但是，测量的时间应该有所不同。
The timing of the measurement of biological samples (e.g. biomarkers) is not always standardized. Biomarkers are the focus of many recent studies and treatments. The purpose of this study was to determine the timing of the release of beta-endorphin (BE), a possible biomarker, after exposure to pain and/or handling stress in order to standardize measurements. Mouse plasma was collected for BE analysis following handling i.e. being picked up by the investigator, exposure to a painful (55°C hot-plate), or exposure to a nonpainful stimulus (room temperature hot-plate). The groups exposed to either a painful or nonpainful stimulus released BE in response to the stimulus, but the duration of the response was longer in mice exposed to a painful stimulus than in mice exposed to a nonpainful stimulus. The BE in the mice exposed to a nonpainful stimulus peaked at 1min and returned to baseline levels by 5min while the BE response of the mice exposed to a painful stimulus peaked at 10min and remained elevated for 25min. The results of this study indicate that BE can be a biomarker for pain and handling stress, however, the timing of the measurement should differ.