MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis
复制标题
DOI：
10.1186/1471-2180-9-145
复制
发表时间：
2009-07-20
期刊：
影响因子：
4.2
通讯作者：
Vergnaud, Gilles
中科院分区：
文献类型：
--
作者：
Maquart, Marianne;Le Fleche, Philippe;Vergnaud, Gilles
背景：自1994年以来，布鲁氏菌菌株已从多种海洋哺乳动物中分离出来。它们是目前公认的两个布鲁氏菌新种，即B。pinnipedialis为鳍足类分离物，B.鲸类分离物的ceti与宿主偏好和特定的表型和分子标记一致。为了研究海洋哺乳动物布鲁氏菌分离株与陆地哺乳动物布鲁氏菌分离株之间的遗传关系，我们在这项研究中应用了多位点VNTR（可变串联重复数）分析（MLVA）方法。一个以前发表的测定，包括16个位点（MLVA-16），已被证明是高度相关的和有效的分型和聚类布鲁氏菌菌株从动物和人类originatedweredused.Results：294海洋哺乳动物布鲁氏菌菌株收集在欧洲沃茨从173动物和人从新西兰分离大概从海洋起源进行了调查MLVA-16。海洋哺乳动物布鲁氏菌分离株与公认的陆地哺乳动物布鲁氏菌种和生物群不同，对应于3个主要相关群，其中一个是B。ceti菌株是B. pinnipedialis菌株和最后一种由人分离株组成。在B。CETI组中，鉴定了3个亚群，区分了海豚、小须鲸和鼠海豚分离物的一个群和主要由海豚分离物组成的两个群。这些结果与使用其他表型或分子方法或不同的VNTR基因座组的已发表分析一致。B。pinnipedialis群也可分为3个亚群，其中一个亚群仅由来自冠海豹的分离物组成（Cystophora cristata）和另外两个包含其他海豹物种分离株。对来自欧洲沃茨的大量海洋哺乳动物布鲁氏菌分离株的聚类分析大大加强了目前对这两个物种种群结构的看法，以及它们相对于其余布鲁氏菌属的相对位置。MLVA-16是一种快速、高分辨、重复性好的方法，可用于布鲁氏菌菌株（包括海洋哺乳动物分离株）的分类。布鲁氏菌2009 MLVA-16基因分型数据库可在http://mlva.u-psud.fr/上获得，该数据库提供了目前公认的所有9种布鲁氏菌的详细覆盖范围。
Background: Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used.Results: 294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals (Cystophora cristata) and the two others comprising other seal species isolates.Conclusion: The clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with respect to the rest of the Brucella genus. MLVA-16 is confirmed as being a rapid, highly discriminatory and reproducible method to classify Brucella strains including the marine mammal isolates. The Brucella2009 MLVA-16 genotyping database available at http://mlva.u-psud.fr/ is providing a detailed coverage of all 9 currently recognized Brucella species.