Integrated multi-omics approach revealed cellular senescence landscape.
Integrated multi-omics approach revealed cellular senescence landscape.
复制标题
综合多组学方法揭示细胞衰老景观
DOI：
10.1093/nar/gkac885
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发表时间：
2022-10-28
影响因子：
14.9
通讯作者：
Wang, Peichang
中科院分区：
文献类型：
--
作者：
Song, Qiao;Hou, Yuli;Zhang, Yiyin;Liu, Jing;Wang, Yaqi;Fu, Jingxuan;Zhang, Chi;Cao, Min;Cui, Yuting;Zhang, Xiaomin;Wang, Xiaoling;Zhang, Jingjing;Liu, Congcong;Zhang, Yingzhen;Wang, Peichang
摘要细胞衰老是一种复杂的多因素生物现象，在衰老及其相关疾病中起着重要作用。在这个过程中，衰老细胞经历了基因表达的改变和染色质结构的重塑。然而，使用集成的多组学方法对衰老的表观遗传学图景进行的研究有限。在本研究中，我们对不同的衰老类型进行了ATAC-SEQ、RNA-SEQ和CHIP-SEQ，以揭示衰老的图景，并找出主要的调控元件。我们还获得了34个关键基因，推测NAT1、Pbx1和RRM2相互作用，可能是衰老和衰老相关疾病的潜在标志。综上所述，我们的工作为研究细胞衰老中的可及性动力学和转录调控提供了平台。这项技术在不同类型的衰老中的应用使我们能够识别负责转录实质性调控的调控元件，为深入了解衰老的分子机制提供了线索。
Abstract Cellular senescence is a complex multifactorial biological phenomenon that plays essential roles in aging, and aging-related diseases. During this process, the senescent cells undergo gene expression altering and chromatin structure remodeling. However, studies on the epigenetic landscape of senescence using integrated multi-omics approaches are limited. In this research, we performed ATAC-seq, RNA-seq and ChIP-seq on different senescent types to reveal the landscape of senescence and identify the prime regulatory elements. We also obtained 34 key genes and deduced that NAT1, PBX1 and RRM2, which interacted with each other, could be the potential markers of aging and aging-related diseases. In summary, our work provides the landscape to study accessibility dynamics and transcriptional regulations in cellular senescence. The application of this technique in different types of senescence allows us to identify the regulatory elements responsible for the substantial regulation of transcription, providing the insights into molecular mechanisms of senescence.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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