Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunits
Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunits
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DOI：
10.1007/s00251-003-0582-9
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发表时间：
2003-07
期刊：
影响因子：
3.2
通讯作者：
Y. Yoshiura;I. Kiryu;A. Fujiwara;H. Suetake;Yuzuru Suzuki;T. Nakanishi;M. Ototake
中科院分区：
文献类型：
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作者：
Y. Yoshiura;I. Kiryu;A. Fujiwara;H. Suetake;Yuzuru Suzuki;T. Nakanishi;M. Ototake
我们分离并鉴定了河豚、红鳍东方鲀、哺乳动物白细胞介素 (IL)-12 亚基（IL-12 p35 和 IL-12 p40）的直系同源物的 cDNA 和基因。 FuguIL-12亚基的推导氨基酸序列显示出与哺乳动物IL-12亚基的同源性（p35：50.4-58.0％相似性；p40：51.2-55.4％相似性）。系统发育分析证实FuguIL-12 p35 和p40 基因与其哺乳动物对应谱系聚类。每个FuguIL-12亚基基因的基因组组织与相应的小鼠IL-12亚基基因的基因组组织相似，尽管Fugu基因由于内含子尺寸小而非常紧凑。比较基因组分析显示Fugu和人之间IL-12 p35和p40区域内的保守同线性，表明FuguIL-12 p35和p40基因分别是哺乳动物IL-12 p35和p40编码基因的直向同源物。在淋巴组织和几种非淋巴组织中观察到 IL-12 p35 mRNA 的表达，而 IL-12 p40 mRNA 的表达是组成型的并且几乎无处不在。在脾脏和头肾中，IL-12 p35 的表达是由聚核糖肌苷多核糖胞苷酸 [poly(I:C)] 诱导的，而不是由脂多糖 (LPS) 诱导的，而 IL-12 p40 的表达是组成型的，对聚(I:C) 和 LPS 均无反应。这些结果表明 IL-12 水平受 IL-12 p35 mRNA 的产生调节，并表明鱼类中的 IL-12 可能参与抗病毒防御。这是第一份关于非哺乳动物脊椎动物中 IL-12 亚基 cDNA 和基因的鉴定和表征的报告。
We have isolated and characterized cDNAs and genes for pufferfish,Fugu rubripes,(Fugu) orthologues of mammalian interleukin (IL)-12 subunits (IL-12 p35 and IL-12 p40). The deduced amino acid sequences of theFuguIL-12 subunits showed homology with mammalian IL-12 subunits (p35: 50.4–58.0% similarity; p40: 51.2–55.4% similarity). Phylogenetic analysis confirmed thatFuguIL-12 p35 and p40 genes cluster with their mammalian counterpart lineages. The genomic organization of each of theFuguIL-12 subunit genes is similar to that of the corresponding mouse IL-12 subunit genes, although theFugugenes are very compact due to small intron size. Comparative genomic analysis showed conserved syntenies within the IL-12 p35 and p40 regions betweenFuguand human, indicating that theFuguIL-12 p35 and p40 genes are orthologues for mammalian IL-12 p35 and p40 encoding genes, respectively. Expression of IL-12 p35 mRNA was observed in lymphoid tissues and several non-lymphoid tissues, while expression of IL-12 p40 mRNA was constitutive and nearly ubiquitous. In the spleen and head kidney, expression of IL-12 p35 was induced by polyriboinosinic polyribocytidylic acid [poly(I:C)] and not by lipopolysaccharide (LPS), while expression of IL-12 p40 was constitutive and unresponsive to both poly(I:C) and LPS. These results indicate that IL-12 levels are regulated by production of IL-12 p35 mRNA and suggest that IL-12 in fish may be involved in antiviral defense. This is the first report of the identification and characterization of IL-12 subunit cDNAs and genes in a non-mammalian vertebrate.