Increase of CGRP Expression in Motor Endplates Within Fore and Hind Limb Muscles of the Degenerating Muscle Mouse (Scn8admu)
Increase of CGRP Expression in Motor Endplates Within Fore and Hind Limb Muscles of the Degenerating Muscle Mouse (Scn8admu)
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退化肌肉小鼠 (Scn8admu) 前肢和后肢肌肉运动终板中 CGRP 表达的增加
DOI：
10.1007/s10571-010-9601-1
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发表时间：
2010
影响因子：
4
通讯作者：
H. Ichikawa
中科院分区：
文献类型：
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作者：
Tadasu Sato;Y. Shimizu;Mitsuhiro Kano;Toshihiko Suzuki;H. Kanetaka;L. W. Chu;P. D. Côté;H. Shimauchi;H. Ichikawa
降钙素基因相关肽（CGRP）在具有电压门控钠通道基因escn8a零突变的退行性肌肉（dmu）小鼠的前、后肢骨骼肌以及口腔和颅面区域的分布进行了检测。在野生型小鼠的肢体、口腔和颅面肌中，只有少数运动终板具有cgrp免疫反应性。然而，在肱三头肌、肱二头肌、肱肌和腓肠肌中出现了许多cgrp免疫反应性运动终板。骨骼肌运动终板的cgrp免疫反应密度也因突变而升高。在这些肌肉中，可以检测到肌纤维萎缩，肌肉组织中细胞核密度增加。而在指深屈肌、指浅屈肌和比目鱼肌以及口腔和颅面肌中，cgrp免疫反应性的分布几乎不受突变的影响。野生型和家鼠的肌纤维形态和细胞核分布也很相似。大鼠腰椎脊髓脊髓运动神经元cgrp免疫反应密度增高。这些结果提示，小鼠部分前肢和后肢肌肉萎缩变性可能增加其运动神经元中CGRP的表达。
The distribution of calcitonin gene-related peptide (CGRP) was examined in skeletal muscles of fore and hind limb as well as in oral and cranio-facial regions of thedegenerating muscle(dmu) mouse, which harbours a null mutation in the voltage-gated sodium channel geneScn8a. In limb, oral and cranio-facial muscles of wild type mice, only a few motor endplates contained CGRP-immunoreactivity. However, many CGRP-immunoreactive motor endplates appeared in the triceps brachii muscle, the biceps brachii muscle, the brachialis muscle, and the gastrocnemius muscle ofdmumice. CGRP-immunoreactive density of motor endplates in the skeletal muscles was also elevated by the mutation. In these muscles, the atrophy of muscle fibers could be detected and the density of cell nuclei in the musculature increased. In the flexor digitorum profundus muscle, the flexor digitorum superficialis muscle, and the soleus muscle as well as in oral and cranio-facial muscles, however, the distribution of CGRP-immunoreactivity was barely affected by the mutation. The morphology of muscle fibers and the distribution of cell nuclei within them were also similar in wild type anddmumice. In the lumbar spinal cord ofdmumice, CGRP-immunoreactive density of spinal motoneurons increased. These findings suggest that the atrophic degeneration in some fore and hind limb muscles ofdmumice may increase CGRP expression in their motoneurons.
Fukunishi, Y. 等人：“用辣根过氧化物酶细胞内染色的猫舌下运动神经元的树突结构的定量分析”J.
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