Biological activity of a K-ras mutant that contains the 12R/59T/116Y mutations.
Biological activity of a K-ras mutant that contains the 12R/59T/116Y mutations.
复制标题
包含 12R/59T/116Y 突变的 K-ras 突变体的生物活性。
DOI：
10.1016/0304-3835(93)90202-k
复制
发表时间：
1993
期刊：
影响因子：
9.7
通讯作者：
Kuzumaki,N
中科院分区：
文献类型：
--
作者：
Ogiso,Y;Hwang,YW;Shih,TY;Kuzumaki,N
Abstract The 12R/59T/116Y mutations have been shown to confer a dominant negative activity on H-ras oncogene (H-ras 116Y). To determine whether this event is unique for H-ras, we introduced the same mutations into K-ras oncogene. This mutant, K-ras 116Y, suppressed transformed phenotypes induced by overexpression of H-ras proto-oncogene. NIH3T3 cells expressing K-ras 116Y were resistant to transformation by v-fes oncogene. Analysis of chimaeras between H-and K-ras 116Y showed that the C-terminal variable region determines the level of suppressor activity. These results suggest that these mutations are applicable to other GDP MGTP binding proteins.
