CHEMICAL BASIS OF ROUGH AND SMOOTH VARIATION IN MYCOBACTERIA
CHEMICAL BASIS OF ROUGH AND SMOOTH VARIATION IN MYCOBACTERIA
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DOI：
10.1128/jb.171.6.3465-3470.1989
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发表时间：
1989-06-01
影响因子：
3.2
通讯作者：
BRENNAN, PJ
中科院分区：
文献类型：
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作者：
BELISLE, JT;BRENNAN, PJ
堪萨斯分枝杆菌的粗糙和光滑的菌落变种进行了比较，相对于表面糖脂组成。天然糖脂抗原的薄层层析、组分糖的气相层析以及通过菌落斑点印迹酶联免疫吸附测定和免疫金标记用适当的单克隆抗体原位探测证实了所有的M.具有光滑菌落形态的Kansasii菌株在其表面上含有最近描述的含海藻糖的脂寡糖，而所有粗糙变体都没有这种表面抗原。然而，所有的菌株，粗糙和光滑，含有另一种糖脂，M。堪萨斯特有的酚糖脂。其他人先前的研究表明，M.在实验感染的小鼠中，kansasii比光滑变体持续时间更长。因此，这项研究可能会提供一些深入了解的化学基础的问题，在某些分枝杆菌的发病机制。
Rough and smooth colony variants of Mycobacterium kansasii were compared with respect to surface glycolipid composition. Thin-layer chromatography of the native glycolipid antigens, gas chromatography of the constituent sugars, and in situ probing with an appropriate monoclonal antibody by colony dot blot enzyme-linked immunosorbent assay and immunogold labeling demonstrated that all M. kansasii strains of smooth colony morphology contain on their surfaces the recently described trehalose-containing lipooligosaccharides, whereas all rough variants were devoid of such surface antigens. Yet all strains, rough and smooth, contained another glycolipid, the M. kansasii-specific phenolic glycolipid. Previous studies by others had shown that the rough forms of M. kansasii persist longer than smooth variants in experimentally infected mice. Therefore, this study may provide some insight into the question of the chemical basis of pathogenesis in certain mycobacteria.