DNA barcoding is a useful tool for the identification of marine fishes from Japan
DNA barcoding is a useful tool for the identification of marine fishes from Japan
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DNA 条形码是鉴定日本海洋鱼类的有用工具
DOI：
10.1016/j.bse.2010.12.017
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发表时间：
2011-02-01
影响因子：
1.6
通讯作者：
Hanner, Robert
中科院分区：
文献类型：
--
作者：
Zhang, Jun-Bin;Hanner, Robert
利用日本158种海水鱼类细胞色素氧化酶亚基I基因(COI)的229个DNA序列，对DNA条形码进行物种鉴定的效果进行了检验。种间平均遗传距离是种内平均遗传距离的60倍，木村双参数(K2P)遗传距离在同种间平均为17.6%，在同种间仅为0.3%。种内K2P距离和种间K2P距离之间没有重叠，所有序列在相邻连接的树状图中形成了物种单位。通过检索生命条码数据系统(BLOD)，还检测到两个物种(Kyphosus vaigiensis和Pterocaesio digram ma)的杂交现象。DNA条形码为鱼类识别提供了一种新的方法。皇冠版权所有(C)2011由爱思唯尔有限公司出版。保留所有权利。
In this study, 229 DNA sequences of cytochrome oxidase subunit I gene (COI) from 158 marine fishes of Japan were employed to test the efficacy of species identification by DNA barcoding. The average genetic distance was 60-fold higher between species than within species, as Kimura two parameter (K2P) genetic distances averaged 17.6% among congeners and only 0.3% among conspecifics. There were no overlaps between intraspecific and interspecific K2P distances, and all sequences formed species units in the neighbor-joining dendrogram. Hybridization phenomena in two species (Kyphosus vaigiensis and Pterocaesio digramma) were also detected through searches in Barcode of Life Data Systems (BOLD). DNA barcoding provides a new way for fish identification. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.