Automated multiwell plate STORM: towards open source super-resolved high content analysis
Automated multiwell plate STORM: towards open source super-resolved high content analysis
复制标题
自动化多孔板 STORM：迈向开源超分辨高内涵分析
DOI：
10.1117/12.2526940
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Görlitz F
中科院分区：
文献类型：
--
作者：
Görlitz F
简介：在超分辨显微镜（SRM）方法中，单分子定位显微镜技术，如光激活定位显微镜（PALM）[1]和随机光学重建显微镜（STORM）[2]，使成像超过经典衍射极限，以获得新的见解，在亚细胞生物过程中相对简单的仪器。这导致了许多低成本的仪器，例如用于STORM显微镜[3-6]，其可以受益于用于单分子定位显微镜（SMLM）数据分析的一系列软件工具[7]。我们的低成本“easySTORM”方法[4]使用多模二极管激光器和光纤实现dSTORM [8]，提供视场直径高达~ 125 μm的STORM图像，使用μManager [9]控制图像数据采集，使用ThunderSTORM [10]分析SMLM数据。我们和其他人[11，12]致力于开发用于高含量分析（HCA）的自动化SMLM，其能够快速成像样品阵列，允许对可能在标记和生物异质性方面变化的样品进行统计分析，并实现中等通量筛选应用。
Introduction: Among super-resolved microscopy (SRM) methods, single molecule localisation microscopy techniques, such as photo-activated localisation microscopy (PALM)[l] and stochastic optical reconstruction microscopy (STORM)[2], enable imaging beyond the classical diffraction limit to gain new insights in subcellular biological processes with relatively simple instrumentation. This has led to a number of low-cost instruments, eg for STORM microscopy [3-6], which can benefit from an array of software tools for the single molecule localisation microscopy (SMLM) data analysis [7]. Our low-cost “easySTORM" approach [4] implements dSTORM [8] with multimode diode lasers and optical fibres to provide STORM images with fields of view up to~ 125 μm diameter using μManager [9] to control the image data acquisition and ThunderSTORM [10] to analyse the SMLM data. We and others [11, 12] are motivated to develop automated SMLM for high content analysis (HCA) that enable rapid imaging of sample arrays, allows statistical analysis of samples that may vary in terms of labelling and biological heterogeneity and enable moderate throughput screening applications.
影响因子：
2.9
作者：
Holm, Thorge;Klein, Teresa;Sauer, Markus
通讯作者：
Sauer, Markus