Clock genes outside the suprachiasmatic nucleus involved in manifestation of locomotor activity rhythm in rats
Clock genes outside the suprachiasmatic nucleus involved in manifestation of locomotor activity rhythm in rats
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DOI：
10.1111/j.1460-9568.2000.01313.x
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发表时间：
2000-12-01
影响因子：
3.4
通讯作者：
Honma, K
中科院分区：
文献类型：
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作者：
Masubuchi, S;Honma, S;Honma, K
甲基苯丙胺(MAP)的慢性治疗使大鼠的运动活动节律从明暗周期中分离出来。当活动节律与明暗周期完全相反时，通过原位杂交检测时钟基因(rPer1，rPer2，rBMAL1，rClock)在几个脑结构中的表达，以及松果体和血浆褪黑素的水平。MAP处理组大鼠视交叉上核(SCN)中rPer1的表达表现出与对照组基本相同的昼夜节律。MAP处理的大鼠松果体和血浆褪黑激素的昼夜节律没有明显变化。然而，在尾壳核(CPU)和顶叶皮质中检测到的rPer1、rPer2和rBMAL1表达的强劲昼夜节律在MAP处理组大鼠与对照组大鼠相比完全颠倒，表明与SCN节律去同步。在伏隔核和扣带皮质时钟基因表达的昼夜节律中没有观察到这种去同步化。在MAP处理的大鼠中，CPU和顶叶皮质的rClock表达昼夜节律没有倒转。这些结果表明，大鼠的运动节律是由SCN外的起搏器直接驱动的，参与了CPU和顶叶皮质的rPer1、rPer2和rBMAL1。
Chronic treatment of methamphetamine (MAP) in rats desynchronized the locomotor activity rhythm from the light-dark cycle. When the activity rhythm was completely phase-reversed with respect to a light dark-cycle, 24 h profiles were examined for the clock gene (rPer1, rPer2, rBMAL1, rClock) expressions in several brain structures by in situ hybridization, and for the pineal as well as plasma melatonin levels. In the MAP-treated rats, the rPer1 expression in the suprachiasmatic nucleus (SCN) showed a robust circadian rhythm which was essentially identical to that in the control rats. Circadian rhythms in pineal as well as plasma melatonin were not changed significantly in the MAP-treated rats. However, robust circadian rhythms in the rPer1, rPer2 and rBMAL1 expressions detected in the caudate-putamen (CPU) and parietal cortex were completely phase-reversed in the MAP-treated rats, compared with those in the control rats, indicating desynchronization from the SCN rhythm. Such desynchronization was not observed in the circadian rhythms of clock gene expression in the nucleus accumbens and cingulate cortex. The circadian rClock expression rhythm in the MAP-treated rats was not phase-reversed in the CPU and parietal cortex. These findings indicate that the locomotor activity rhythm in rats is directly driven by the pacemaker outside the SCN, in which rPer1, rPer2 and rBMAL1 in the CPU and parietal cortex are involved.