Studies on riboflavin metabolism in the rat. VI. Properties of radioactive metabolites excreted after administration of riboflavin-2- 14 C.
Studies on riboflavin metabolism in the rat. VI. Properties of radioactive metabolites excreted after administration of riboflavin-2- 14 C.
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大鼠核黄素代谢的研究。
DOI：
10.1111/j.1600-0773.1971.tb00648.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
S. Christensen
中科院分区：
文献类型：
--
作者：
S. Christensen
研究了核黄素-214 C给药后大鼠尿液和粪便中排泄的放射性代谢产物。尿液纸层析（正丁醇：乙酸：水4：1：5）表明存在5种放射性代谢物。平均Rf值分别为0.10、0.20、0.42、0.53和0.63。用尿素酶孵育尿液表明存在微量放射性尿素。粪便中含有尿液中发现的两种代谢物。代谢产物Rf 0.63被鉴定为光色素。其优势的粪便中的意义进行了讨论。除了代谢产物Rf 0.53（其激发光谱已显示）外，代谢产物在水性介质中不发荧光。它们比核黄素更易溶于水。在Sephadex G-25柱上，它们比核黄素洗脱得更快。水解实验表明，没有一个代谢产物是核黄素的共轭物。
The radioactive metabolites excreted in the urine and faeces after administration of riboflavin-214C to rats were investigated. Paper chromatography (n-butanol:acetic acid:water 4:1:5) of urine indicated the presence of five radioactive metabolites. The average Rf-values were 0.10, 0.20, 0.42, 0.53 and 0.63 respectively. Incubation of urine with urease indicated the presence of minute amounts of radioactive urea. The faeces contained two of the metabolites found in the urine. Metabolite Rf 0.63 was identified as lumichrome. The significance of its predominance in the faeces is discussed. Apart from the metabolite Rf 0.53, the excitation spectrum of which is presented, the metabolites did not fluoresce in an aqueous medium. They were more soluble in water than riboflavin. On a Sephadex G-25 column they were eluted more rapidly than riboflavin. Hydrolysis experiments showed that none of the metabolites were conjugates of riboflavin.