Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin.
Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin.
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DOI：
10.1016/j.jbiotec.2011.08.013
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发表时间：
2011-12-10
影响因子：
4.1
通讯作者：
Linhardt, Robert J.
中科院分区：
文献类型：
--
作者：
Wang, Zhenyu;Li, Jennifer;Cheong, Samantha;Bhaskar, Ujjwal;Akihiro, Onishi;Zhang, Fuming;Dordick, Jonathan S.;Linhardt, Robert J.
化学酶法合成生物工程肝素的化学步骤已被检查和优化统计使用响应面法。试验采用四因素二水平完全析因设计和三因素Box-Behnken设计。目的是建立一种方法来制备所需的N-乙酰基含量，数均分子量的N-磺基，N-乙酰基肝素原，并在最大产率通过控制反应物的浓度，反应时间和反应温度。利用所建立的响应面模型预测了从大肠杆菌中制备N-磺基，N-乙酰肝素原的最佳反应条件。大肠杆菌产生不同分子量的肝素原起始材料。
The chemical step in the chemoenzymatic synthesis of bioengineered heparin has been examined and optimized statistically using a response surface methodology. A four factor, two level full factorial design experiment and a three factor Box-Behnken design were carried out. The goal was to establish a method to prepare N-sulfo, N-acetyl heparosan of the desired N-acetyl content, number average molecular weight, and in maximum yield by controlling the reactant concentrations, reaction time and reaction temperature. The response surface models obtained were used to predict the reaction conditions required to optimally prepare N-sulfo, N-acetyl heparosan from E. coli generated heparosan starting material of different molecular weights.
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影响因子：
3.8
作者：
Wang, Zhenyu;Ly, Mellisa;Zhang, Fuming;Zhong, Weihong;Suen, Amy;Hickey, Anne Marie;Dordick, Jonathan S.;Linhardt, Robert J.
通讯作者：
Linhardt, Robert J.
影响因子：
15
作者：
Zhang, Zhenqing;McCallum, Scott A.;Xie, Jin;Nieto, Lidia;Corzana, Francisco;Jimenez-Barbero, Jesus;Chen, Miao;Liu, Jian;Linhardtt, Robert J.
通讯作者：
Linhardtt, Robert J.
影响因子：
5
作者：
Wang, Zhenyu;Yang, Bo;Zhang, Zhenqing;Ly, Mellisa;Takieddin, Majde;Mousa, Shaker;Liu, Jian;Dordick, Jonathan S.;Linhardt, Robert J.
通讯作者：
Linhardt, Robert J.
影响因子：
46.9
作者：
Guerrini, Marco;Beccati, Daniela;Sasisekharan, Ram
通讯作者：
Sasisekharan, Ram
影响因子：
7.4
作者：
Aslan, N.;Cebeci, Y.
通讯作者：
Cebeci, Y.