[Relationships between decreased LAMC3 and poor prognosis in ovarian cancer].
[Relationships between decreased LAMC3 and poor prognosis in ovarian cancer].
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DOI：
10.3760/cma.j.cn112141-20210426-00230
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发表时间：
2021-07-25
期刊：
影响因子：
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通讯作者：
Yin, F J
中科院分区：
文献类型：
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作者：
Lei, S M;Liu, X;Yin, F J
目的：探讨层粘连蛋白亚单位γ3(LAMC3)表达与卵巢癌(OC)预后的关系。方法：采用免疫组织化学链霉亲和素-过氧化物酶-生物素连接法检测LAMC3蛋白的表达。应用癌症基因组图谱(TCGA)队列和临床蛋白质组肿瘤分析联盟(CPTAC)中的基因表达和相关临床数据分析基因和蛋白表达与临床结果的相关性。采用Pearson CHI2检验(双侧)评价LAMC3与临床病理因素的相关性。用Kaplan-Meier图计算生存概率和显着性。利用LAMC3共表达基因丰富的生物通路的功能聚集性来探讨LAMC3可能导致预后不良的可能机制。结果：根据216例卵巢癌组织(包括208例肿瘤和8例正常卵巢组织)和51例卵巢癌组织(包括24例化疗耐药组织和27例敏感组织)的免疫组化结果和CPTAC(包括100例原发肿瘤和25例正常卵巢组织)的蛋白表达数据，结果表明：卵巢癌组织中LAMC3的蛋白表达明显低于正常组织，晚期组织中LAMC3的蛋白表达低于早期组织，耐药组织中LAMC3的蛋白表达水平低于敏感组织(均P
Objective: To investigate the correlations of laminin subunit gamma 3 (LAMC3) expression with prognosis of ovarian cancer (OC). Methods: LAMC3 protein expression was measured using immunohistochemical streptavidin-peroxidase-biotin connection method (IHC). Gene expression and related clinical data in the cancer genome atlas (TCGA) cohort and clinical proteomic tumor analysis consortium (CPTAC) were applied to analyse the correlation between gene and protein expressions and clinical outcomes. Correlations between LAMC3 and clinicopathological factors were evaluated using the Pearson chi2 test (2-sided). The probability of survival and significance was calculated using the Kaplan-Meier plot. The functional clustering of biological pathways enriched from co-expressed genes of LAMC3 was used to explore the possible mechanisms that LAMC3 might contribute to poor prognosis. Results: Based on the IHC results of 216 OC tissues or ovaries (including 208 tumors and 8 normal tissues) and 51 OC tissues (including 24 chemotherapy-resistant and 27 sensitive tissues), and the protein expression data from CPTAC (including 100 primary tumors and 25 normal tissues), the results showed that the protein expression of LAMC3 was significantly decreased in OC tissues compared with normal, decreased in advanced-stage tissues compared with early-stage tissues, and decreased in drug-resistant tissues compared with sensitive tissues (all P