Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome
Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome
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DOI：
10.2152/jmi.53.117
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发表时间：
2006-02-01
影响因子：
0.7
通讯作者：
Nakahori, Yutaka
中科院分区：
文献类型：
--
作者：
Kinoshita, Keigo;Shinka, Toshikatsu;Nakahori, Yutaka
Y染色体热休克转录因子（HSFY）位于Y染色体上无精子因子（AZF）的三个候选区域之一。我们和其他人已经发现，一些无精子症男性缺失Y染色体的区域，包括HSFY。以前，我们发现小鼠HSFY样序列[mHSFYL（Riken cDNA 4933413 G11 Rik）]，即小鼠HSFY的直向同源物，仅在睾丸中表达。编码HSFY和mHSFYL中假定的DNA结合结构域的序列在其他哺乳动物如狗、牛和鸡中发现。为了详细阐明mHSFYL在睾丸中的表达，我们进行了原位杂交。mHSFYL主要在圆形精子细胞中表达。此外，我们澄清了在COS 1细胞中的HA或GFP标记的蛋白质的mHSFYL的细胞内分布。HA-mHSFYL和GFP-mHSFYL均位于细胞核内。我们的研究结果表明，HSFY/mHSFYL可能具有进化保守的精子发生功能。
Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence [mHSFYL (Riken cDNA 4933413G11Rik)], which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA- or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.