Trypsin-catalyzed peptide synthesis and various p-guanidinophenyl esters as acyl donors.
Trypsin-catalyzed peptide synthesis and various p-guanidinophenyl esters as acyl donors.
复制标题
胰蛋白酶催化的肽合成和各种对胍基苯酯作为酰基供体。
DOI：
10.1248/cpb.44.1585
复制
发表时间：
1996
影响因子：
1.7
通讯作者：
K. Tanizawa
中科院分区：
文献类型：
--
作者：
H. Sekizaki;K. Itoh;E. Toyota;K. Tanizawa
以N-α-叔丁氧羰基氨基酸和肽的对胍基苯酯为酰基供体，研究了胰蛋白酶催化的肽合成。对反应条件进行了优化，考察了有机溶剂、pH值、受体浓度等因素对反应的影响。该方法特别适用于制备各种含D-氨基酸的肽。所得产物的酶促水解可忽略不计。
Trypsin-catalyzed peptide synthesis has been studied by using p-guanidinophenyl esters of N alpha-(tert-butyloxycarbonyl)amino acid and peptide as acyl donor components. The reaction conditions were optimized for organic solvents, pH, and concentration of acceptor. The method was especially useful for the preparation of various peptides containing D-amino acids. The enzymatic hydrolysis of the resulting products was negligible.