Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin region (eastern Poland).
Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin region (eastern Poland).
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卢布林地区（波兰东部）蓖麻硬蜱中伯氏疏螺旋体基因种的流行率。
DOI：
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发表时间：
2006
影响因子：
1.7
通讯作者：
J. Dutkiewicz
中科院分区：
文献类型：
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作者：
E. Cisak;A. Wójcik;N. Stojek;J. Chmielewska;J. Zwoliński;A. Buczek;J. Dutkiewicz
该研究的目的是确定从卢布林地区（波兰东部）林区采集的蓖麻硬蜱中 3 个伯氏疏螺旋体基因种的流行率。通过聚合酶链式反应 (PCR) 对来自 6 个地区的 1,813 只蓖麻蜱进行了检查，以确定是否存在伯氏疏螺旋体 (B.b.s.l.)。对从 4 个地区收集的另一组 438 只蓖麻蜱进行了检查，以确定是否存在 B.b.s.l.。通过在 BSKH 液体培养基上培养，通过 PCR 确认，并确定疏螺旋体属 (Borrelia spp) 的存在。通过暗视野显微镜（DFM）。通过巢式 PCR 在 113 只蜱裂解液中测定了伯氏疏螺旋体基因种（Borrelia burgdorferi sensu stricto、Borrelia afzelii 和 Borrelia garinii），显示存在 B.b.s.l。 （在 PCR 中或在培养物和 PCR 中）。通过 PCR 检查，5.4% 的蓖麻蜱显示出 B.b.s.l. 的存在。脱氧核糖核酸。雌性感染率最高（12.1%），雄性感染率较低（6.0%），若虫感染率最低（1.7%）（p < 0.001）。蓖麻蜱 B.b.s.l 的最低感染率。培养测定的感染率为 3.4%，而 DFM 测定的形态类似疏螺旋体的活动螺旋体蜱的最低感染率为 11.2%。在 6 个检查地区中的 5 个地区收集的蜱虫中发现了所有 3 个正在调查的伯氏疏螺旋体基因种的存在。在 81.4% 的感染蜱中，仅观察到 1 个基因种的单一感染，而在 16.8% 和 1.8% 的感染蜱中，分别检测到 2 种或 3 个基因种的双重感染。严格伯氏疏螺旋体（Borrelia burgdorferi sensu stricto）是所有检查的蜱阶段和地区中的主要基因种，无论是单一感染还是混合感染，并且在总共 62.8% 的蓖麻蜱中发现了 B.b.s.l. 感染。阿氏疏螺旋体 (Borrelia afzelii) 和加氏疏螺旋体 (Borrelia garinii) 的发生率较低，分别在 39.8% 和 17.8% 的感染蜱中观察到。
The objective of the study was to determine the prevalence rate of 3 Borrelia burgdorferi genospecies in Ixodes ricinus ticks collected from wooded areas of the Lublin region (eastern Poland). A group of 1,813 I. ricinus ticks from 6 districts were examined for the presence of Borrelia burgdorferi sensu lato (B.b.s.l.) by polymerase chain reaction (PCR). Another group of 438 I. ricinus ticks collected from 4 districts were examined for the presence of B.b.s.l. by culture on BSKH liquid medium confirmed by PCR, and for the presence of Borrelia spp. by dark field microscopy (DFM). Borrelia burgdorferi genospecies (Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii) were determined by nested-PCR in 113 ticks lysates showing presence of B.b.s.l. (in PCR or in culture and PCR). 5.4 % of I. ricinus ticks examined by PCR showed the presence of B.b.s.l. DNA. The infection rate was highest in females (12.1 %), lower in males (6.0 %) and the lowest in nymphs (1.7 %) (p < 0.001). The minimum infection rate of I. ricinus ticks with B.b.s.l. determined by culture was 3.4 %, whereas the minimum infection rate of ticks with motile spirochetes morphologically resembling Borrelia spp., determined by DFM, amounted to 11.2 %. The presence of all 3 Borrelia burgdorferi genospecies under investigation was found in ticks collected from 5 out of 6 examined districts. In 81.4 % of infected ticks only single infection with 1 genospecies was observed, while coinfections with 2 or 3 genospecies were detected respectively in 16.8 % and 1.8 % of infected ticks. Borrelia burgdorferi sensu stricto was the dominant genospecies in all examined tick stages and districts, both in single infections and in coinfections, and found in a total of 62.8 % of I. ricinus ticks infected with B.b.s.l. Borrelia afzelii and Borrelia garinii were less frequent and observed in respectively 39.8 % and 17.8 % of infected ticks.