Retention mechanisms in reversed-phase chromatography. Stationary phase bonding density and solute selectivity.
Retention mechanisms in reversed-phase chromatography. Stationary phase bonding density and solute selectivity.
复制标题
反相色谱中的保留机制。
DOI：
10.1016/s0021-9673(00)94287-0
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Dorsey,JG
中科院分区：
文献类型：
--
作者：
Sentell,KB;Dorsey,JG
小非极性溶质的色谱选择性已确定为单体十八烷基固定相键合密度的函数，范围为 1.74–4.07 μmol/m2。苯基或形状选择性随着键合密度的增加而增加，而亚甲基选择性保持大致恒定。这些发现与 Dill 的平均场统计热力学理论一致，该理论预测固定相链密度的增加应导致各向异性链有序性的增加和溶质形状选择性的增加。这些研究进一步证明，分配而非吸附是反相液相色谱中小非极性分子的主要保留模式。
Chromatographic selectivity for small, non-polar solutes has been determined as a function of monomeric octadecyl stationary phase bonding density over the range 1.74–4.07 μmol/m2. Phenyl or shape selectivity increases with increasing bonding density, whereas methylene selectivity remains approximately constant. These findings are in agreement with the mean field statistical thermodynamic theory of Dill, which predicts that increased stationary phase chain density should lead to increased anisotropic chain ordering and increased solute-shape selectivity. These studies provide further evidence that partitioning, not adsorption, is the dominant mode of retention for small, non-polar molecules in reversed-phase liquid chromatography.