Evaluation of optical coherence tomography for the measurement of the effects of activators and anticoagulants on the blood coagulation in vitro.
Evaluation of optical coherence tomography for the measurement of the effects of activators and anticoagulants on the blood coagulation in vitro.
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光学相干断层扫描测量活化剂和抗凝剂对体外血液凝固影响的评价
DOI：
10.1109/tbme.2013.2245329
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发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Xu X;Geng J;Liu G;Chen Z
利用光学相干断层扫描（OCT）研究了人体血液凝固过程中的光学特性，并在我们以前的工作中开发了由1/e光穿透深度（d1/e）与时间的关系推导出的凝固时间参数。在这项研究中，为了了解一种新的光学相干断层扫描测试是否可以表征体外不同治疗下的血液凝固过程，研究了两种不同的激活剂（钙离子和凝血酶）和抗凝剂，即，评价了不同浓度的乙酰水杨酸（阿萨，一种众所周知的药物阿司匹林）和美拉曲肽（一种直接凝血酶抑制剂）。采用中心波长为1300 nm的扫频光源OCT系统，在静态条件下对体外血液凝固过程进行了检测。d1/e的动态研究揭示了由于钙离子和凝血酶诱导的凝血的典型行为，并且凝血时间是浓度依赖性的。剂量依赖性阿萨和melagliptin延长凝血时间。阿萨和Melaglavin对血液凝固的影响不同。正如预期的那样，通过OCT测量，melaglavin在抗凝方面比阿萨有效得多。OCT测定似乎是一种用于测量凝血以评估活化剂和抗凝剂的作用的简单方法，其可用于活化剂和抗凝剂筛选。
Optical properties of human blood during coagulation was studied using optical coherence tomography (OCT) and the parameter of clotting time derived from the 1/e light penetration depth (d1/e) vs. time was developed in our previous work. In this study, in order to know if a new optical coherence tomography test can characterize the blood coagulation process under different treatments in vitro, the effects of two different activators (calcium ions and thrombin) and anticoagulants, i.e., acetylsalicylic acid (ASA, a well-known drug aspirin) and melagatran (a direct thrombin inhibitor), at various concentrations are evaluated. A swept source OCT system with a 1300 nm center wavelength is used for detecting the blood coagulation process in vitro under a static condition. A dynamic study of the d1/e reveals a typical behavior due to coagulation induced by both calcium ions and thrombin, and the clotting time is concentration-dependent. Dose-dependent ASA and melagatran prolong the clotting times. ASA and melagatran have different effects on blood coagulation. As expected, melagatran is much more effective than ASA in anticoagulation by the OCT measurements. The OCT assay appears to be a simple method for the measurement of blood coagulation to assess the effects of activators and anticoagulants, which can be used for activator and anticoagulant screening.