Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix
Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix
复制标题
单一人乳头瘤病毒 16 或 52 持续感染病例的初始病毒载量与宫颈后期细胞病理学发现的进展相关
DOI：
10.1002/jmv.23709
复制
发表时间：
2013
影响因子：
12.7
通讯作者：
Masuzaki H
中科院分区：
文献类型：
--
作者：
Hamaguchi D;Miura K;Abe S;Kinoshita A;Miura S;Yamasaki K;Yoshiura K;Masuzaki H
本研究的目的是探讨单个人乳头瘤病毒(HPV)16或52持续感染的病毒载量与宫颈细胞病理学进展的关系。对305例致癌性HPV阳性妇女在3~6个月内复查宫颈细胞学和HPV基因分型。其中单一HPV52持续感染24例，单一HPV16持续感染24例。晚期细胞病理学结果显示进展的病例被定义为进展组，而那些没有变化或退化者被定义为非进展组。用实时定量聚合酶链式反应测定HPVDNA相对载量，并相对人白蛋白(ALB)DNA进行表达。比较两组间的差异。进展组HPV52DNA载量中位数为2.211，非进展组为0.022(Mann-WhitneyU检验，P= 0.003)。进展组HPV16DNA载量中位数为4.206，非进展组为0.103(P= 0.001)。用实时定量方法评估HPV52和16DNA载量可能是识别宫颈细胞学病理进展的高危女性的有用的短期标记物。维罗尔。85：2093-2100,2013。©2013 Wiley Permials，Inc.
The aim of this study was to investigate the relationship between viral load in single human papillomavirus (HPV) 16 or 52 persistent infection and the progression of later cytopathological findings in the uterine cervix. Cervical cytology and HPV genotyping tests were repeated within 3–6 months in 305 women with oncogenic HPV. Twenty‐four cases of single HPV 52 persistent infection and 24 cases of single HPV 16 persistent infection were identified. Cases with later cytopathological findings showing progression were defined as the progression group, while those with no change or regression were the non‐progression group. Relative HPV DNA loads were determined by quantitative real‐time polymerase chain reaction and expressed relative to human albumin (ALB) DNA. Differences between the two groups were evaluated. The median relative HPV 52 DNA load was 2.211 in the progression group and 0.022 in the non‐progression group (Mann–WhitneyU‐test,P= 0.003). The median relative HPV 16 DNA load was 4.206 in the progression group and 0.103 in the non‐progression group (P= 0.001). HPV 52 and 16 DNA loads assessed by quantitative real‐time methods may be useful short‐term markers for identifying women at high risk for progression of cervical cytological pathology.J. Med. Virol. 85:2093–2100, 2013.© 2013 Wiley Periodicals, Inc.