Melanomacrophage response and hepatic histopathologic biomarkers in the guppy Poecilia reticulata exposed to iron oxide (maghemite) nanoparticles
Melanomacrophage response and hepatic histopathologic biomarkers in the guppy Poecilia reticulata exposed to iron oxide (maghemite) nanoparticles
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DOI：
10.1016/j.aquatox.2018.02.014
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发表时间：
2018-05-01
影响因子：
4.5
通讯作者：
Rocha, Thiago Lopes
中科院分区：
文献类型：
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作者：
Qualhato, Gabriel;Teixeira de Saboia-Morais, Simone Maria;Rocha, Thiago Lopes
虽然氧化铁纳米颗粒（IONP）已被广泛应用于纳米医学和纳米修复，其对水生生物的生态毒理学效应尚不清楚。在这项研究中，黑色素巨噬细胞中心（MMC）的反应和肝脏组织病理学生物标志物进行了研究，在雌性孔雀鱼，孔雀鱼，暴露于柠檬酸盐功能化的IONP（γ-Fe 2 O3）在环境相关的铁浓度（0.3毫克L-1）超过21天。在实验开始时以及暴露3、7、14和21天后收集动物。与未暴露的相比，暴露于IONP的孔雀鱼显示MMC的数量、面积和周长增加，尤其是暴露7天后。结果显示，暴露于IONP 7天后，鱼的组织病理学变化频率增加，如微泡和大泡脂肪变性、黑色素巨噬细胞聚集体、渗出物和出血灶。急性（3和7天）和长期（14和21天）暴露于IONP的网状拟青霉诱导了与循环障碍和炎症反应相关的高组织病理学指标。结果表明，MMC反应和组织病理学指标是指示IONP的环境影响的重要生物标志物，证实了孔雀鱼是IONP的生态毒性的目标。
Although iron oxide nanoparticles (IONPs) have been widely used in nanomedicine and nanoremediation, their ecotoxicological effects on aquatic organisms remain unclear. In this study, the melanomacrophage center (MMC) response and hepatic histopathologic biomarkers were investigated in female guppies, Poecilia reticulate, exposed to citrate-functionalized IONPs (gamma-Fe2O3) at an environmentally relevant iron concentration (0.3 mg L-1) over 21 days. The animals were collected at the beginning of the experiment and after 3, 7, 14, and 21 days of exposure. Guppies exposed to IONPs showed increases in the number, area, and perimeter of MMC when compared with the unexposed ones, especially after 7 days of exposure. The results showed an increase in the frequency of histopathologic changes in fish after 7 days of exposure to IONPs, such micro- and macro- vesicular steatosis, melanomacrophage aggregates, exudate, and hemorrhagic foci. The acute (3 and 7 days) and long-term (14 and 21 days) exposure of P. reticulata to IONPs induced high histopathologic indexes associated with circulatory disorders and inflammatory responses. Results showed that the MMC response and histopathologic index are important biomarkers to indicate the environmental impact of IONPs, confirming that the guppy P. reticulata is a target of ecotoxicity of IONPs.