Role of actin in the cAMP-dependent activation of sodium/glucose cotransporter in renal epithelial cells
Role of actin in the cAMP-dependent activation of sodium/glucose cotransporter in renal epithelial cells
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DOI：
10.1016/j.bbamem.2005.02.008
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发表时间：
2005-06-01
影响因子：
3.4
通讯作者：
Takagi, K
中科院分区：
文献类型：
--
作者：
Ikari, A;Harada, H;Takagi, K
We examined whether actin filaments are involved in the cAMP-dependent activation of a high affinity sodium/glucose cotransporter (SGLT1) using epithelial expression systems. The expression of enhanced green fluorescent protein-tagged SGLT1 (EGFP-SGLT1) in Madin-Darby canine kidney (MDCK) cells was revealed by Western blotting and confocal laser microscopy. 8-Br-cAMP, a membrane permeable cAMP analog, enhanced [C-14]-alpha-methyl glucopyranoside ([C-14]-AMG) uptake. Both basal and 8-Br-cAMP-elicited [C-14]-AMG uptakes were inhibited by N-(2 {[3-(4-bromophenyl)-2 -propenyl]-amino}-ethyl)-5-isoquinolinesulfonamide (H-89), a protein kinase A inhibitor, and cytochalasin D, an actin filament formation inhibitor. Furthermore, cytochalasin D inhibited the distribution of EGFP-SGLT1 at the apical surface. These results suggest that the EGFP-SGLT1 protein is functionally expressed in the apical membrane of MDCK cells, and is up-regulated by a cAMP-dependent pathway requiring intact actin filaments. (c) 2005 Elsevier B.V All rights reserved.
