Phytochemical glyceollins, isolated from soy, mediate antihormonal effects through estrogen receptor α and β
Phytochemical glyceollins, isolated from soy, mediate antihormonal effects through estrogen receptor α and β
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DOI：
10.1210/jc.86.4.1750
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发表时间：
2001-04-01
影响因子：
5.8
通讯作者：
McLachlan, JA
中科院分区：
文献类型：
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作者：
Burow, ME;Boue, SM;McLachlan, JA
类黄酮植物化学物质的类黄酮家族，特别是从大豆中提取的类黄酮。已受到关注，因为它们的雌激素活性以及它们对人类健康和疾病的影响。除了这些黄酮类化合物，其他植物化学物质，包括植物芪，肠内酯，木脂素，具有内分泌活性。大豆和其他植物中这些化合物的类型和数量受组成型表达和胁迫诱导的生物合成的控制。因此，大豆食品的健康益处可能取决于这些食品中各种植物活性植物化学物质的含量。目的是确定独特的大豆植物化学物质，以前没有评估雌激素或抗雌激素活性，在这里，我们描述了增加的生物合成的大豆植物抗毒素化合物。大豆抗毒素，在胁迫条件下生长的大豆植物中。与所观察到的香豆雌酚、大豆苷元和染料木黄酮的雌激素效应相反，我们观察到大豆抗素对ER信号传导的显著抗雌激素效应，这与MCF-7细胞中17 β-雌二醇诱导的增殖的相当抑制相关。进一步的评估显示，在瞬时转染的HEK 293细胞中，对ER α的拮抗作用比对ER β的拮抗作用更大。竞争结合测定揭示大豆抗毒素对ER α us的更大亲和力。ERP，其与较高浓度的大豆抗毒素对ER α信号传导的更大抑制相关。总之，我们描述了被称为glyceolins的植物防御素化合物，其在HEK 293和MCF-7细胞中对ER表现出独特的拮抗作用。大豆抗毒素以及其他植物抗毒素化合物可能是大豆食品对健康影响的重要组成部分。
The flavonoid family of phytochemicals, particularly those derived from soy. has received attention regarding their estrogenic activity as well as their effects on human health and disease. In addition to these flavonoids other phytochemicals, including phytostilbene, enterolactone, and lignans, possess endocrine activity. The types and amounts of these compounds in soy and other plants are controlled by both constitutive expression and stress-induced biosynthesis. The health benefits of soy-based foods may, therefore, be dependent upon the amounts of the various hormonally active phytochemicals within these foods. The aim was to identify unique soy phytochemicals that had not been previously assessed for estrogenic or antiestrogenic activity, Here we describe increased biosynthesis of the isoflavonoid phytoalexin compounds. glyceollins, in soy plants grown under stressed conditions. In contrast to the observed estrogenic effects of coumestrol, daidzein, and genistein, we observed a marked antiestrogenic effect of glyceollins on ER signaling, which correlated with a comparable suppression of 17 beta -estradiol-induced proliferation in MCF-7 cells. Further evaluation revealed greater antagonism toward ER alpha than ER beta in transiently transfected HEK 293 cells. Competition binding assays revealed a greater affinity of glyceollins for ERa us. ERP, which correlated to greater suppression of ER alpha signaling with higher concentrations of glyceollins. In conclusion, we describe the phytoalexin compounds known as glyceollins, which exhibit unique antagonistic effects on ER in both HEK 293 and MCF-7 cells. The glyceollins as well as other phytoalexin compounds may represent an important component of the health effects of soy-based foods.