A new peptaibol, RK-026A, from the soil fungus Trichoderma sp. RK10-F026 by culture condition-dependent screening.
A new peptaibol, RK-026A, from the soil fungus Trichoderma sp. RK10-F026 by culture condition-dependent screening.
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DOI：
10.1093/bbb/zbaa051
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Mira Syahfriena Amir Rawa;T. Nogawa;Akiko Okano;Y. Futamura;Takemichi Nakamura;H. Wahab;H. Osada
中科院分区：
文献类型：
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作者：
Mira Syahfriena Amir Rawa;T. Nogawa;Akiko Okano;Y. Futamura;Takemichi Nakamura;H. Wahab;H. Osada
从真菌木霉属RK 10-F026中分离出一种新的peptaibol，RK-026 A（1），沿着的还有黄绿菌素B（2）、丙甲霉素II（3）和作为细胞毒性化合物的多孢菌素B（4），这些化合物是通过对来自5种不同培养条件的MS数据进行主成分分析而选出的。通过NMR和HR-MS分析，包括详细的MS/MS碎片研究，确定1的结构为新的在第18位残基处含有Glu而不是Gln的atroviridin B衍生物。1显示对K562白血病细胞的细胞毒性，IC 50值为4.1 µm。
A new peptaibol, RK-026A (1) was isolated from a fungus, Trichoderma sp. RK10-F026, along with atroviridin B (2), alamethicin II (3), and polysporin B (4) as a cytotoxic compound, which was selected by principal component analysis of the MS data from 5 different culture conditions. The structure of 1 was determined as a new atroviridin B derivative containing Glu at the 18th residue instead of Gln by NMR and HR-MS analyses including the investigation of detailed MS/MS fragmentations. 1 showed cytotoxicity toward K562 leukemia cells at an IC50 value of 4.1 µm.