Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body
Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body
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DOI：
10.7554/elife.43079
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发表时间：
2019-05-21
期刊：
影响因子：
7.7
通讯作者：
Jefferis, Gregory S. X. E.
中科院分区：
文献类型：
--
作者：
Dolan, Michael-John;Frechter, Shahar;Jefferis, Gregory S. X. E.
动物对包括嗅觉在内的各种感官刺激表现出与生俱来的行为。在果蝇中，一个更高的嗅觉中心，侧角（LH），与先天行为有关。然而，我们对LH的结构和功能的理解是不足的，这在很大程度上是由于该区域缺乏稀疏的神经遗传学工具。我们生成了一组分裂gal4驱动系，提供了82种LH细胞类型的遗传通路。我们使用这些数据来创建LH的解剖和神经递质图，并将其与EM连接组学数据联系起来。我们发现-30%的LH投射与蘑菇体（嗅觉学习和记忆的场所）的输出收敛。利用光遗传激活，我们确定了驱动价行为或特定运动程序变化的LH细胞类型。总之，我们已经生成了操纵和绘制LH神经元的资源，为先天和习得嗅觉行为的电路基础提供了新的见解。
Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic tools for this region. We generate a collection of split-GAL4 driver lines providing genetic access to 82 LH cell types. We use these to create an anatomical and neurotransmitter map of the LH and link this to EM connectomics data. We find -30% of LH projections converge with outputs from the mushroom body, site of olfactory learning and memory. Using optogenetic activation, we identify LH cell types that drive changes in valence behavior or specific locomotor programs. In summary, we have generated a resource for manipulating and mapping LH neurons, providing new insights into the circuit basis of innate and learned olfactory behavior.