Birth of germline chimeras by transfer of chicken embryonic germ (EG) cells into recipient embryos
Birth of germline chimeras by transfer of chicken embryonic germ (EG) cells into recipient embryos
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DOI：
10.1002/mrd.10304
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发表时间：
2003-08-01
影响因子：
2.5
通讯作者：
Han, JY
中科院分区：
文献类型：
--
作者：
Park, TS;Hong, YH;Han, JY
本研究首次报道了将鸡胚生殖细胞（EG）移植到不同品系的受体胚胎中，获得鸡胚系嵌合体。从带有1/1基因的28期白色来航鸡（WL）胚胎中取出性腺组织细胞，通过传代培养建立EG细胞。在原代培养过程中，间质细胞中的性腺原始生殖细胞（gPGCs）在培养7 d后开始形成集落，与对照组相比差异有显著性（P
This study reports for the first time the production of chicken germline chimeras by transfer of embryonic germ (EG) cells into recipient embryos of different strain. EG cells were established by the subculture of gonadal tissue cells retrieved from stage 28 White Leghorn (WL) embryos with 1/1 gene. During primary culture (PO), gonadal primordial germ cells (gPGCs) in the stromal cells began to form colonies after 7 days in culture with significant (P