Primary root growth in Arabidopsis thaliana is inhibited by the miR159 mediated repression of MYB33, MYB65 and MYB101
Primary root growth in Arabidopsis thaliana is inhibited by the miR159 mediated repression of MYB33, MYB65 and MYB101
复制标题
拟南芥初生根生长受到 miR159 介导的 MYB33、MYB65 和 MYB101 抑制的抑制
DOI：
10.1016/j.plantsci.2017.06.008
复制
发表时间：
2017
期刊：
影响因子：
5.2
通讯作者：
Fengning Xiang
中科院分区：
文献类型：
--
作者：
Tao Xue;Zhenhua Liu;Xuehuan Dai;Fengning Xiang;Fengning Xiang
器官生长是一个以细胞增殖和分化为基础的基本发育过程。植物根的生长由根分生组织的活性维持，该过程部分地由各种转录因子控制。在这里，miR159已被确定为根生长的转录后抑制因子，其基础是miR159abdouble突变体比野生型发育了更大的分生组织，并且形成了更长的根。在突变体中，MYB 33、MYB 65和MYB 101转录本的丰度显著增加。当MYB 33、MYB 65和MYB 101分别被miR159抗性形式smMYB 33、mMYB 65和mMYB 101取代时，根分生组织同样扩大，主根的生长增强。MYB 65活性通过加速细胞周期促进根分生组织的细胞分裂。这些数据表明，miR159作为主根生长的关键抑制因子，通过其对MYB 65的抑制和随后的细胞周期阻断起作用。
Organ growth is a fundamental developmental process basing on cell proliferation and differentiation. The growth of the plant root is sustained by the activity of the root meristem, a process controlled in part by various transcription factors. Here, themiR159has been identified as a post transcriptional repressor of root growth, on the basis that themir159abdouble mutant developed a larger meristem than did the wild type, and that it formed longer roots. In the mutant, the abundance ofMYB33,MYB65andMYB101transcript was substantially increased. WhenMYB33,MYB65andMYB101were replaced by themiR159-resistant formsmMYB33, mMYB65andmMYB101respectively, the root meristem was similarly enlarged and the growth of the primary root enhanced.MYB65activity promoted cell division in the root meristem by accelerating the cell cycle. The data suggest thatmiR159acts as a key repressor of the primary root's growth, acting through its repression ofMYB65and consequent blocking of the cell cycle.