Transcription Factor AhR Regulates Glutathione S-Transferases Conferring Resistance to lambda-Cyhalothrin in Cydia pomonella
Transcription Factor AhR Regulates Glutathione S-Transferases Conferring Resistance to lambda-Cyhalothrin in Cydia pomonella
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DOI：
10.1021/acs.jafc.3c00002
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发表时间：
2023-03-21
影响因子：
6.1
通讯作者：
Yang, Xue-Qing
中科院分区：
文献类型：
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作者：
Hu, Chao;Liu, Yu-Xi;Yang, Xue-Qing
芳基碳氢化合物受体（AhR）通过调节解毒网络来增强昆虫对杀虫剂的抵抗力。我们之前的研究已经证实，细胞色素P450单加氧酶（P450s）和谷胱甘肽S-转移酶（GSTs）的过度表达与苹果蠹蛾对高效氯氟氰菊酯的抗性有关。在这里，我们报道 CpAhR 调节 GST 和 P450 基因的表达，从而赋予抗性。表达模式表明，CpAhR 的表达受到 λ-三氯氟氰菊酯暴露的高度诱导，并在 λ-三氯氟氰菊酯抗性群体中上调。 CpAhR 的 RNA 干扰 (RNAi) 会降低关键耐药相关基因（CpGSTe3、CpCYP9A121 和 CpCYP9A122）的表达以及 GST 酶的活性，从而降低对高效氯氟氰菊酯的耐受性。此外，β-萘黄酮是一种新型 AhR 激动剂，首次被证明可有效增加 CpAhR 表达和幼虫对高效氯氟氰菊酯的耐受性。这些结果表明，CpAhR 调节关键解毒基因的表达和 GST 活性，导致苹果蠹蛾对高效氯氟氰菊酯产生抗性。
Aryl hydrocarbon receptor (AhR) enhances insect resistance to insecticides by regulating the detoxification network. Our previous studies have confirmed that overexpressions of cytochrome P450 monooxygenases (P450s) and glutathione S- transferases (GSTs) are involved in lambda-cyhalothrin resistance in Cydia pomonella. Here, we report that CpAhR regulates the expression of GST and P450 genes, thus conferring resistance. Expression patterns indicated that the expression of CpAhR was highly induced by lambda-cyhalothrin exposure and upregulated in a lambda-cyhalothrin-resistant population. RNA interference (RNAi) of CpAhR decreases the expression of key resistance-related genes (CpGSTe3, CpCYP9A121, and CpCYP9A122) and the activity of the GST enzyme, reducing the tolerance to lambda-cyhalothrin. Furthermore, beta-naphthoflavone, a novel agonist of AhR, was first proven to be effective in increasing CpAhR expression and larval tolerance to lambda-cyhalothrin. These results demonstrate that CpAhR regulates the expression of key detoxifying genes and GST activity, resulting in the development of resistance to lambda- cyhalothrin in C. pomonella.