High-level Sp1 is Associated with Proliferation, Invasion, and Poor Prognosis in Astrocytoma
High-level Sp1 is Associated with Proliferation, Invasion, and Poor Prognosis in Astrocytoma
复制标题
DOI：
10.1007/s12253-018-0422-8
复制
发表时间：
2019-07-01
影响因子：
2.8
通讯作者：
Kwan, Aij-Lie
中科院分区：
文献类型：
--
作者：
Chen, Yi-Ting;Tsai, Hung-Pei;Kwan, Aij-Lie
星形细胞瘤是成人中最常见和最致命的原发性脑肿瘤。 IV级胶质母细胞瘤通常对目前可用的手术、化疗和放疗治疗无效。已知特异性蛋白 1 (Sp1) 转录因子可调节许多癌症的肿瘤发生。本研究的目的是探讨Sp1蛋白在星形细胞瘤癌变中的临床病理作用。本研究分析了 2002 年至 2012 年期间在高雄医学大学附属医院治疗的 98 例星形细胞瘤病例。通过卡方检验、Kaplan-Meier 分析和 Cox 回归分析来分析与 Sp1 相关的临床病理参数。比较non-siRNA组和Sp1 siRNA组的体外增殖、侵袭和迁移。在用Sp1 siRNA处理的胶质母细胞瘤细胞中，还使用Western blot检测Sp1、Ki-67、VEGF、cyclin D1、E-cadherin、cleaved caspase-3和Bax蛋白的表达。 Sp1 的表达与 WHO 分级 (p=0.005) 和总生存时间 (p
Astrocytoma is the most common and the most lethal primary brain tumor in adults. Grade IV glioblastoma is usually refractory to currently available surgical, chemotherapeutic, and radiotherapeutic treatments. The Specificity protein 1 (Sp1) transcription factor is known to regulate tumorigenesis in many cancers. The aim of this study was to investigate the clinicopathologic role of Sp1 protein in the carcinogenesis of astrocytoma. This study analyzed 98 astrocytoma cases treated at Kaohsiung Medical University Hospital during 2002-2012. Clinicopathologic parameters associated with Sp1 were analyzed by chi-square test, Kaplan-Meier analysis, and Cox regression analyses. In vitro proliferation, invasion, and migration were compared between non-siRNA groups and Sp1 siRNA groups. In glioblastoma cells treated with Sp1 siRNA, Western blot was also used to detect expressions of Sp1, Ki-67, VEGF, cyclin D1, E-cadherin, cleaved caspase-3 and Bax proteins. Expression of Sp1 was significantly associated with WHO grade (p=0.005) and with overall survival time (p