Histone and TK0471/TrmBL2 form a novel heterogeneous genome architecture in the hyperthermophilic archaeon Thermococcus kodakarensis
Histone and TK0471/TrmBL2 form a novel heterogeneous genome architecture in the hyperthermophilic archaeon Thermococcus kodakarensis
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DOI：
10.1091/mbc.e10-08-0668
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发表时间：
2011-02-01
影响因子：
3.3
通讯作者：
Takeyasu, Kunio
中科院分区：
文献类型：
--
作者：
Maruyama, Hugo;Shin, Minsang;Takeyasu, Kunio
与细菌和真核生物不同，细菌是生物的第三个领域。古细菌的DNA复制、转录和翻译机制与真核生物更为相似，而参与代谢过程的基因则与细菌更为相似。我们在这里报告，TK 0471/TrmB-like 2（TrmBL 2），除了组蛋白，是一种新型的丰富的染色体蛋白的模式广古菌Thermococcus kodakarensis。T.科达卡菌可以分成富含组蛋白的区域，其中遗传物质呈现“串珠状”外观，或者富含TK 0471/TrmBL 2的区域，其中它呈现厚的纤维结构。TK 0471/TrmBL 2与编码区和基因间区结合，并在与启动子区结合时抑制转录。这些结果表明，古细菌染色体被组织成异质结构，TK 0471/TrmBL 2作为一个通用的染色体蛋白，以及一个全球性的转录抑制因子。
Being distinct from bacteria and eukaryotes, Archaea constitute a third domain of living things. The DNA replication, transcription, and translation machineries of Archaea are more similar to those of eukaryotes, whereas the genes involved in metabolic processes show more similarity to their bacterial counterparts. We report here that TK0471/TrmB-like 2 (TrmBL2), in addition to histone, is a novel type of abundant chromosomal protein in the model euryarchaeon Thermococcus kodakarensis. The chromosome of T. kodakarensis can be separated into regions enriched either with histone, in which the genetic material takes on a "beads-on-a-string" appearance, or with TK0471/TrmBL2, in which it assumes a thick fibrous structure. TK0471/TrmBL2 binds to both coding and intergenic regions and represses transcription when bound to the promoter region. These results show that the archaeal chromosome is organized into heterogeneous structures and that TK0471/TrmBL2 acts as a general chromosomal protein as well as a global transcriptional repressor.