The composition of a primary T cell response is largely determined by the timing of recruitment of individual T cell clones.
The composition of a primary T cell response is largely determined by the timing of recruitment of individual T cell clones.
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原代T细胞反应的组成在很大程度上取决于单个T细胞克隆的募集时间。
DOI：
10.1084/jem.189.10.1591
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发表时间：
1999-05-17
期刊：
影响因子：
--
通讯作者：
Abastado JP
中科院分区：
文献类型：
--
作者：
Bousso P;Levraud JP;Kourilsky P;Abastado JP
原发性T细胞应答依赖于抗原特异性T细胞前体的募集和增殖。每个单个T细胞克隆的扩增程度可能取决于（a）其在免疫前的频率，（B）其增殖能力，和（c）其首次遇到其同源抗原的时间。在这份报告中，我们分析了这些参数中的每一个的相对贡献的形成免疫库的T细胞反应特异性的表位170-179来自HLA-Cw 3和提出的Kd。通过半脾切除术，我们比较了同一动物的免疫和幼稚库，发现免疫前所有扩增T细胞克隆的频率极低。特别是，最扩展的克隆没有来自高频率的前体。此外，发现募集的T细胞以相同的速率增殖，而不管它们的T细胞抗原受体序列如何。最后，我们发现只有在早期时间点遇到抗原的T细胞才是特异性应答的重要部分。因此，T细胞克隆对免疫应答的贡献主要由其进入免疫库的时间决定，即，抗原接触后第一次细胞分裂的时间。
Primary T cell responses rely on the recruitment and proliferation of antigen-specific T cell precursors. The extent of expansion of each individual T cell clone may depend on (a) its frequency before immunization, (b) its proliferative capacity, and (c) the time at which it first encounters its cognate antigen. In this report, we have analyzed the relative contribution of each of these parameters to the shaping of immune repertoires in the T cell response specific for the epitope 170-179 derived from HLA-Cw3 and presented by Kd. By means of hemisplenectomy, we compared immune and naive repertoires in the same animal and found that the frequency of all expanded T cell clones was extremely low before immunization. In particular, the most expanded clones did not derive from high-frequency precursors. In addition, recruited T cells were found to proliferate at the same rate, irrespective of their T cell antigen receptor sequence. Finally, we showed that only T cells that encounter the antigen at early time points account for a significant part of the specific response. Therefore, the contribution of a T cell clone to the immune response is mostly determined by the time of its entry into the immune repertoire, i.e., the time of first cell division after antigen encounter.