CONSTITUTIVE EXPRESSION OF THE GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR GENE IN ACUTE MYELOBLASTIC-LEUKEMIA
CONSTITUTIVE EXPRESSION OF THE GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR GENE IN ACUTE MYELOBLASTIC-LEUKEMIA
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DOI：
10.1172/jci112769
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发表时间：
1987-01-01
影响因子：
15.9
通讯作者：
GRIFFIN, JD
中科院分区：
文献类型：
--
作者：
YOUNG, DC;WAGNER, K;GRIFFIN, JD
用北方印迹法研究了粒细胞-巨噬细胞集落刺激因子（GM-CSF）基因在正常人造血细胞和一系列白血病中的表达。在活化的T细胞中检测到GM-CSF信使（m）RNA，但在正常骨髓细胞、单核细胞或非活化的T细胞中未检测到。相反，22例急性髓细胞白血病中有11例的白血病细胞表达GM-CSF转录本。在这11种白血病中的6种的条件化上清液中检测到生物活性CSF。GM-CSF基因的表达在“普通”（前B）急性淋巴细胞白血病（11例检测）或慢性髓细胞白血病（4例检测）中未检测到。这些结果表明，GM-CSF基因在AML患者的一个子集中组成型表达，并进一步表明该基因的表达可能有助于AML的异常生长特性。
Expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene was studied by Northern blot analysis in normal human hematopoietic cells and a series of leukemias. GM-CSF messenger (m)RNA was detected in activated T cells, but not in normal bone marrow cells, monocytes, or nonactivated T cells. In contrast, leukemic cells from 11 of 22 cases of acute myeloblastic leukemia expressed GM-CSF transcripts. Biologically active CSF was detected in supernatant conditioned by 6 of these 11 leukemias. Expression of the GM-CSF gene was not detected in "common" (pre-B) acute lymphoblastic leukemia (11 cases tested) or chronic myeloid leukemia (4 cases tested). These results show that the GM-CSF gene is constitutively expressed in a subset of patients with AML, and further suggest that expression of this gene could contribute to the abnormal growth properties characteristic of AML.