MOLECULAR AND BIOCHEMICAL-CHARACTERIZATION OF DNOS - A DROSOPHILA CA2+ CALMODULIN-DEPENDENT NITRIC-OXIDE SYNTHASE
MOLECULAR AND BIOCHEMICAL-CHARACTERIZATION OF DNOS - A DROSOPHILA CA2+ CALMODULIN-DEPENDENT NITRIC-OXIDE SYNTHASE
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DOI：
10.1073/pnas.92.20.9072
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发表时间：
1995-09-26
影响因子：
11.1
通讯作者：
TULLY, T
中科院分区：
文献类型：
--
作者：
REGULSKI, M;TULLY, T
一氧化氮（NO）是一种细胞间信使，参与哺乳动物生理学的各个方面，从血管舒张和巨噬细胞毒性到神经元传递，NO由L-精氨酸通过NO合酶（NOS）合成。在这里，我们报告的果蝇NOS基因，dNOS，位于细胞学位置32 B的克隆。dNOS cDNA编码的蛋白质分子量为152 kDa，与大鼠神经元NOS具有43%的氨基酸序列同一性。与哺乳动物NOS一样，DNOS蛋白含有钙调素、FMN、EAD和NADPH的推定结合位点，DNOS活性在细胞培养中表达时依赖于Ca 2 +/钙调素。dNOS也存在一种与脊椎动物神经元NOS相同的RNA剪接模式。这些结构和功能的观察表明，NOS在脊椎动物和无脊椎动物之间具有显著的保守性。
Nitric oxide (NO) is an intercellular messenger involved with various aspects of mammalian physiology ranging from vasodilation and macrophage cytotoxicity to neuronal transmission, NO is synthesized from L-arginine by NO synthase (NOS). Here, we report the cloning of a Drosophila NOS gene, dNOS, located at cytological position 32B. The dNOS cDNA encodes a protein of 152 kDa, with 43% amino acid sequence identity to rat neuronal NOS. Like mammalian NOSs, DNOS protein contains putative binding sites for calmodulin, FMN, EAD, and NADPH, DNOS activity is Ca2+/calmodulin dependent when expressed in cell culture. An alternative RNA splicing pattern also exists for dNOS, which is identical to that for vertebrate neuronal NOS, These structural and functional observations demonstrate remarkable conservation of NOS between vertebrates and invertebrates.