Mouse models of human bladder cancer as a tool for drug discovery.
Mouse models of human bladder cancer as a tool for drug discovery.
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DOI：
10.1002/0471141755.ph1414s49
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发表时间：
2010-06
影响因子：
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通讯作者：
Abate-Shen, Cory
中科院分区：
文献类型：
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作者：
Seager, Catherine;Puzio-Kuter, Anna M;Cordon-Cardo, Carlos;McKiernan, James;Abate-Shen, Cory
肌肉侵袭性膀胱癌是一种致命的疾病，迫切需要有效的新治疗方法。本单元包含适合评估潜在新疗法的小鼠模型的描述。其中包括一种通过将表达Cre重组酶的腺病毒送入膀胱腔而产生的膀胱癌基因工程小鼠模型。还描述了通过将人膀胱肿瘤细胞灌注到免疫缺陷小鼠膀胱腔中创建的原位小鼠模型。还提供了使用这些模型对新化学实体进行临床前评估的协议，以mTOR抑制剂为例。
Muscle-invasive bladder cancer is a deadly condition in dire need of effective new treatments. This unit contains a description of mouse models suitable for the evaluation of potential new therapies. Included is a genetically engineered mouse model of bladder cancer generated by the delivery of an adenovirus expressing Cre recombinase into the bladder lumen. Also described is an orthotopic mouse model created by the instillation of human bladder tumor cells into the bladder lumen of immune deficient mice. Protocols are also provided on the use of these models for the preclinical evaluation of new chemical entities, with mTOR inhibitors shown as an example.