Influence of cell adhesion and spreading on impedance characteristics of cell-based sensors
Influence of cell adhesion and spreading on impedance characteristics of cell-based sensors
复制标题
DOI：
10.1016/j.bios.2007.11.021
复制
发表时间：
2008-03-14
影响因子：
12.6
通讯作者：
Zhang, Miqin
中科院分区：
文献类型：
--
作者：
Asphahani, Fareid;Thein, Myo;Zhang, Miqin
基于细胞的传感器的阻抗测量是用于真实的实时检测和分析细胞对化学和生物试剂的响应的主要表征途径。检测灵敏度和限制取决于传感器阻抗特性，从而取决于细胞图案化技术。这项研究介绍了一种细胞图案化的方法，将细胞结合在金电极的微阵列上，并证明了单细胞图案化可以大大改善基于细胞的传感器的阻抗特性。小鼠成纤维细胞（NIH 3 T3）通过赖氨酸-精氨酸-甘氨酸-天冬氨酸（KRGD）肽介导的天然细胞粘附过程固定在电极上。电极由三种尺寸制成，并且用共价结合或物理吸附的KRGD（c电极或p电极）固定。与连接到p电极的细胞相比，连接到c电极的细胞对于三种电极尺寸分别增加了48.4%、24.2%和19.0%的可测量电信号强度，这表明电极尺寸和表面化学在细胞粘附和扩散中起关键作用，从而在基于细胞的传感器的阻抗特性中起关键作用。在具有与电池尺寸相当的尺寸的c电极上图案化的单电池表现出良好的扩散电池形态，并且基本上优于在其他配置的电极上图案化的电池。(c)2007 Elsevier B. V.保留所有权利。
Impedance measurements of cell-based sensors are a primary characterization route for detection and analysis of cellular responses to chemical and biological agents in real time. The detection sensitivity and limitation depend on sensor impedance characteristics and thus on cell patterning techniques. This study introduces a cell patterning approach to bind cells on microarrays of gold electrodes and demonstrates that single-cell patterning can substantially improve impedance characteristics of cell-based sensors. Mouse fibroblastcells (NIH3T3) are immobilized on electrodes through a lysine-arginine-glycine-aspartic acid (KRGD) peptide-mediated natural cell adhesion process. Electrodes are made of three sizes and immobilized with either covalently bound or physically adsorbed KRGD (c-electrodes or p-electrodes). Cells attached to c-electrodes increase the measurable electrical signal strength by 48.4%, 24.2%, and 19.0% for three electrode sizes, respectively, as compared to cells attached to p-electrodes, demonstrating that both the electrode size and surface chemistry play a key role in cell adhesion and spreading and thus the impedance characteristics of cell-based sensors. Single cells patterned on c-electrodes with dimensions comparable to cell size exhibit well-spread cell morphology and substantially outperform cells patterned on electrodes of other configurations. (c) 2007 Elsevier B.V. All rights reserved.