Phenolic metabolites of anthocyanins following a dietary intervention study in post-menopausal women
Phenolic metabolites of anthocyanins following a dietary intervention study in post-menopausal women
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DOI：
10.1002/mnfr.201300322
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发表时间：
2014-03-01
影响因子：
5.2
通讯作者：
Kay, Colin D.
中科院分区：
文献类型：
--
作者：
de Ferrars, Rachel M.;Cassidy, Aedin;Kay, Colin D.
范围许多研究喂养富含花青素的食物报告有限的生物利用度的母体花青素。本研究探讨的身份和浓度的花青素的酚类代谢产物在human.Methods和resultsAnthocyanin代谢物进行了定量的样品中收集的一个先前进行的12周的接骨木干预研究健康的绝经后妇女。使用HPLC-ESI-MS/MS分析了急性（单次推注）和慢性（12周补充）花青素消耗（500 mg/天）后的个体1，2和3小时推注后尿液样本和合并血浆样本。在尿液和血浆中（包括香草酸，原儿茶酸和苯甲酸的硫酸盐）鉴定了28种花青素代谢物。酚类代谢物在血浆中达到1237 nM的峰值浓度，而花青素缀合物仅达到34 nM的浓度。同样，在尿液中，酚类代谢产物检测浓度为33185 - 2549 nM/mM肌酐，而花青素共轭物达到浓度为548 +/- 219 nM/mM肌酐。没有证据表明，慢性暴露有任何影响的档案或数量的代谢产物回收相对于acuteexposition. ConclusionA广泛的范围内的花青素的酚类代谢产物被确定后接骨木果消费在人类中，包括11个新的代谢产物，这被确定在比他们的母体化合物的浓度高得多。
ScopeNumerous studies feeding anthocyanin-rich foods report limited bioavailability of the parent anthocyanins. The present study explores the identity and concentration of the phenolic metabolites of anthocyanins in humans.Methods and resultsAnthocyanin metabolites were quantified in samples collected from a previously conducted 12-wk elderberry intervention study in healthy post-menopausal women. Individual 1-, 2- and 3-h post-bolus urine samples and pooled plasma samples following acute (single bolus) and chronic (12-wk supplementation) anthocyanin consumption (500 mg/day) were analysed using HPLC-ESI-MS/MS. Twenty-eight anthocyanin metabolites were identified in urine and 21 in plasma (including sulfates of vanillic, protocatechuic and benzoic acid). Phenolic metabolites reached peak concentrations of 1237 nM in plasma, while anthocyanin conjugates only reached concentrations of 34 nM. Similarly, in urine, phenolic metabolites were detected at concentrations of 33185 2549 nM/mM creatinine, while anthocyanin conjugates reached concentrations of 548 +/- 219 nM/mM creatinine. There was no evidence that chronic exposure had any impact on either the profile or quantity of metabolites recovered relative to acute exposure.ConclusionAn extensive range of phenolic metabolites of anthocyanin was identified following elderberry consumption in humans, including 11 novel metabolites, which were identified at much higher concentrations than their parent compounds.