RESPIRATORY METABOLISM OF A PETITE-NEGATIVE YEAST SCHIZOSACCHAROMYCES-POMBE 972H-1
RESPIRATORY METABOLISM OF A PETITE-NEGATIVE YEAST SCHIZOSACCHAROMYCES-POMBE 972H-1
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DOI：
10.1128/jb.104.1.473-481.1970
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发表时间：
1970-01-01
影响因子：
3.2
通讯作者：
LOUIS, C
中科院分区：
文献类型：
--
作者：
HESLOT, H;GOFFEAU, A;LOUIS, C
研究了分裂性、单倍体、“小阴性”酵母裂糖酵母（schizosaccharomyces pombe972h−）的呼吸代谢。葡萄糖和甘油是很好的生长基质，在适当的条件下可被氧化。乳酸盐、乙醇、苹果酸盐和琥珀酸盐被氧化，但它们是生长的不良底物。d-乳酸和丙酮酸既不氧化也不用于生长。在厌氧条件下观察到生长受限。在富氧介质中加入0.3%的kno3可减轻氧的需要量。在葡萄糖抑制浓度下，细胞呼吸在生长过程中持续增加，表明葡萄糖抑制呼吸的存在。还原的烟酰胺腺嘌呤二核苷酸（NADH）、琥珀酸盐、α-甘油磷酸、抗坏血酸盐和四甲基对苯二胺被线粒体部分氧化。抗霉素A和NaCN能抑制NADH和琥珀酸盐的氧化，但鱼藤酮不能，这表明磷酸化位点I不存在，而位点II和III存在。几种线粒体抑制剂对生长和呼吸的影响表明，生长对氧化剂的需求既与呼吸电子传递链的功能无关，也与呼吸能量的形成无关。先前提出的营养“小体”突变体的无活力、葡萄糖对呼吸的抑制缺失以及在厌氧条件下无法生长之间的相关性，因此对s并不严格有效。非洲酒。
The respiratory metabolism ofSchizosaccharomyces pombe972h−, a fission, haplontic, “petite negative” yeast, was studied. Glucose and glycerol are good growth substrates and are oxidized under appropriate conditions.l-Lactate, ethanol, malate, and succinate are oxidized but are poor substrates for growth.d-Lactate and pyruvate are neither oxidized nor used for growth. Limited growth was observed under anaerobic conditions. The addition of 0.3% KNO3to a rich medium relieves the oxygen requirement. A continuous increase of cell respiration during growth on repressive concentration of glucose was observed, suggesting the presence of glucose repression of respiration. Reduced nicotinamide adenine dinucleotide (NADH), succinate, α-glycerophosphate, and ascorbate plus tetramethyl-p-phenylenediamine are oxidized by a mitochondrial fraction. NADH and succinate oxidations are inhibited by antimycin A and NaCN but not by rotenone, suggesting the absence of the phosphorylation site I and the presence of sites II and III. The effects of several mitochondrial inhibitors on growth and respiration indicate that the requirement of an oxidant for growth is related neither to the functioning of the respiratory electron transport chain nor to the formation of respiratory energy. The previously suggested correlations between the nonviability of vegetative “petites” mutants, the absence of repression of respiration by glucose, and the incapacity to grow under anaerobic conditions are thus not strictly valid forS. pombe.