Two Isoforms of Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCH-L1) were Down-Regulated in High Metastatic Potential of Human SN12C Renal Cell Carcinoma Cell Clones
Two Isoforms of Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCH-L1) were Down-Regulated in High Metastatic Potential of Human SN12C Renal Cell Carcinoma Cell Clones
复制标题
泛素羧基末端水解酶同工酶 L1 (UCH-L1) 的两种亚型在人 SN12C 肾细胞癌细胞克隆的高转移潜能中下调
DOI：
10.4172/jpb.s1000158
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Kazuyuki Nakamura
中科院分区：
文献类型：
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作者：
Toshiyuki Tanaka;Y. Kuramitsu;M. Fujimoto;S. Naito;M. Oka;Kazuyuki Nakamura
SN 12 C肾癌细胞克隆为亲本细胞系和3个克隆。SN 12 C亲本细胞系是从人肾细胞癌手术标本中建立的。两个克隆，SN 12 C-clone 2和SN 12 C-PM 6，具有高于亲本细胞系的活性。SN 12 C-克隆4具有比亲本细胞系低的。利用双向凝胶电泳技术，我们检测到8个蛋白质，显示亲本细胞系和高转移克隆之间的差异斑点强度。利用液相色谱-串联质谱法对其中的5个蛋白质进行了鉴定。我们发现两种亚型的UCH-L1蛋白，这是显着下调，在高转移克隆。然而，UCH-L1的下调参与转移的机制仍有待进一步研究。为了阐明UCH-L1蛋白的表达机制，还需要进一步的研究。此外，我们需要研究UCH-L1的下调发生在何种整合机制中。
The SN12C renal cell carcinoma cell clones were parent cell line and 3 clones. SN12C parent cell line was established from a human renal cell carcinoma surgical specimen. The two clones, SN12C-clone 2 and SN12C-PM 6, have higher than the parent cell line. The SN12C-clone 4 has lower than the parent cell line. Using two-dimensional gel electrophoresis, we detected eight proteins showing differential spot intensity between parent cell line and high metastatic clones. And we have identified 5 out of the 8 proteins by using liquid chromatography-tandem mass spectrometry. We found two isoforms of UCH-L1 protein which was shown to be significantly down-regulated in the high metastatic clones. However, the mechanisms of down-regulation of UCH-L1 which were involved in metastasis still remain to be characterized. To clarify the mechanism of UCH-L1 protein expression, further studies will be necessary. Furthermore, we need to examine in what kind of integral mechanism does the down-regulation of UCH-L1 occur.