Neuronal and glial localization of NR1 and NR2A/B subunits of the NMDA receptor in the human cerebral cortex
Neuronal and glial localization of NR1 and NR2A/B subunits of the NMDA receptor in the human cerebral cortex
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DOI：
10.1093/cercor/9.2.110
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发表时间：
1999-03-01
期刊：
影响因子：
3.7
通讯作者：
Ducati, A
中科院分区：
文献类型：
--
作者：
Conti, F;Barbaresi, P;Ducati, A
N-甲基-D-天冬氨酸（NMDA）受体在许多皮质功能中起关键作用，并与多种神经精神疾病有关。在这项研究中，细胞表达的NMDAR 1（NR 1）酸NMDAR 2A和B（NR 2A和B）亚单位，在人类大脑皮层的免疫细胞化学与抗体识别的NR 1或NR 2A和B亚单位的NMDA受体。在额叶（10区和46区）和颞叶（21区）联合皮层和扣带额叶过渡皮层（32区），提示和NR 2A/B免疫反应（ir）是相似的，并定位于所有皮层层的许多神经元。NR 1和NR 2A/B阳性神经元主要为锥体细胞，但也有部分非锥体细胞被标记。电镜观察表明，NR 1和NR 2 A/B ir相似。在所有情况下，标记的树突和树突棘是强烈的。此外，NR 1和NR 2A/B在某些轴突终末的轴浆和远端星形胶质细胞突起中一致地被发现。这项研究表明，许多NMDA受体定位于树突棘，他们也定位于轴突终末和星形胶质细胞的过程。这些研究结果表明，在人类皮层皮层NMDA激活的影响不完全依赖于位于突触后位点的NMDA通道的开放，并且NMDA受体的定位在各种哺乳动物物种中是相似的。
N-Methyl-D-aspartate (NMDA) receptors play a critical role in many cortical functions and are implicated in several neuropsychiatric diseases. In this study, the cellular expression of the NMDAR1 (NR1) acid NMDAR2A and B (NR2A and B) subunits was investigated in the human cerebral cortex by immunocytochemistry with antibodies that recognize the NR1 or the NR2A and B subunits of the NMDA receptor. In frontal (areas 10 and 46) and temporal (area 21) association cortices and the cingulofrontal transition cortex (area 32), hint and NR2A/B immunoreactivity (ir) were similar and were localized to numerous neurons in all cortical layers. NR1- and NR2A/B-positive neurons were mostly pyramidal cells, but some nonpyramidal neurons were also labeled. Electron-microscopic observations showed that NR1 and NR2A/B ir were similar. In all cases, labeling of dendrites and dendritic spines was intense. In addition, both NR1 and NR2A/B were consistently found in the axoplasm of some axon terminals and in distal astrocytic processes. This investigation revealed that numerous NMDA receptors are localized to dendritic spines, and that they are also localized to axon terminals and astrocytic processes. These findings suggest that the effects of cortical NMDA activation in the human cortex do not depend exclusively on the opening of NMDA channels located at postsynaptic sites, and that the localization of NMDA receptors is similar in a variety of mammalian species.