Retinal Pigment Epithelium Expansion Around the Neural Retina Occurs in Two Separate Phases With Distinct Mechanisms
Retinal Pigment Epithelium Expansion Around the Neural Retina Occurs in Two Separate Phases With Distinct Mechanisms
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DOI：
10.1002/dvdy.24525
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发表时间：
2017-08-01
影响因子：
2.5
通讯作者：
McFarlane, Sarah
中科院分区：
文献类型：
--
作者：
Cechmanek, Paula Bernice;McFarlane, Sarah
背景资料：视网膜色素上皮（RPE）是一种特化的单层上皮细胞，其形成围绕神经视网膜的紧密屏障。RPE细胞对于成熟的感光细胞的更新和存活是必不可少的，但是在眼睛发育过程中，最初的RPE细胞群如何在神经视网膜周围扩展却知之甚少。结果如下：在这里，我们的特点的分化，增殖和运动的视网膜色素上皮祖细胞在斑马鱼胚胎视杯形态发生的时期。RPE祖细胞存在于眼囊外翻后不久的背内侧眼囊中。我们定义了两个独立的阶段，允许完全的RPE扩展。第一阶段涉及先前未表征的内眼囊泡小叶中RPE祖结构域的向前扩展，主要由细胞数量的增加驱动。在此阶段，RPE祖细胞开始表达分化标记物。在第二阶段，祖域伸展在背腹和后轴，涉及细胞的运动和形状的变化，并符合视杯形态发生。值得注意的是，RPE扩增不需要细胞分裂。结论：产生覆盖神经视网膜后部的单层上皮的RPE发育发生在由不同机制驱动的两个不同阶段。(C)2017 Wiley Periodicals，Inc.
Background: The retinal pigment epithelium (RPE) is a specialized monolayer of epithelial cells that forms a tight barrier surrounding the neural retina. RPE cells are indispensable for mature photoreceptor renewal and survival, yet how the initial RPE cell population expands around the neural retina during eye development is poorly understood. Results: Here we characterize the differentiation, proliferation, and movements of RPE progenitors in the Zebrafish embryo over the period of optic cup morphogenesis. RPE progenitors are present in the dorsomedial eye vesicle shortly after eye vesicle evagination. We define two separate phases that allow for full RPE expansion. The first phase involves a previously uncharacterized antero-wards expansion of the RPE progenitor domain in the inner eye vesicle leaflet, driven largely by an increase in cell number. During this phase, RPE progenitors start to express differentiation markers. In the second phase, the progenitor domain stretches in the dorsoventral and posterior axes, involving cell movements and shape changes, and coinciding with optic cup morphogenesis. Significantly, cell division is not required for RPE expansion. Conclusions: RPE development to produce the monolayer epithelium that covers the back of the neural retina occurs in two distinct phases driven by distinct mechanisms. (C) 2017 Wiley Periodicals, Inc.