Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens
Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens
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DOI：
10.1016/j.jbiotec.2008.10.011
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发表时间：
2009-03-10
影响因子：
4.1
通讯作者：
Borriss, R.
中科院分区：
文献类型：
--
作者：
Chen, X. H.;Koumoutsi, A.;Borriss, R.
植物相关淀粉液化芽孢杆菌FZB42的基因组含有一系列巨型基因簇，参与合成具有抗真菌、抗菌和杀线虫活性的脂肽和聚酮类化合物。共137kb的5个基因簇SRF、BMY、FEN、NRS、DHB可直接合成环状脂肽表面素、杆菌素、风霉素、所有未知肽和铁铁载体杆菌素。此外，在FZB42中，编码参与合成和输出抗菌二肽杆菌素的酶的一个基因簇也具有功能。Min、Bae和Dfn三个基因簇的总大小为199 kb，可直接合成抗菌作用聚酮大乳素、杆菌烯和难治素，其中FZB42约340 kb，占其总遗传量的8.5%，用于次生代谢物的合成。相反，参与依赖核糖体合成抗生素和其他多肽的基因却很少。除了两个不完整的基因簇直接免疫分生孢菌素和枯草杆菌素外，在培养液中只检测到一种抑制枯草杆菌生长的类似肽的化合物，缺乏替代的西格玛因子W(C)2008 Elsevier B.V.。保留所有权利。
The genome of plant-associated Bacillus amyloliquefaciens FZB42 harbors an array of giant gene clusters involved in synthesis of lipopeptides and polyketides with antifungal, antibacterial and nematocidal activity. Five gene Clusters, srf, bmy, fen, nrs, dhb, covering altogether 137 kb, were shown to direct synthesis of the cyclic lipopeptides surfactin, bacillomycin, fengycin, ail unknown peptide, and the iron-siderophore bacillibactin. In addition, one gene cluster encoding enzymes involved in synthesis and export of the antibacterial dipeptide bacilysin is also functional in FZB42. Three gene clusters, min, bae, and dfn, with a total size of 199 kb were shown to direct synthesis of the antibacterial acting polyketides macrolactin, bacillaene, and difficidin, In total, FZB42 dedicates about 340 kb corresponding to 8.5% of its total genetic capacity, to synthesis of secondary metabolites. On the contrary, genes involved in ribosome-dependent synthesis of lantibiotics and other peptides are scarce. Apart from two incomplete gene clusters directing immunity against mersacidin and subtilin, only one peptide-like compound has been detected in the Culture fluid that inhibits the growth of B. subtilis lacking the alternative sigma factor W. (C) 2008 Elsevier B.V. All rights reserved.