CELLS OF ORIGIN OF ENTORHINAL CORTICAL AFFERENTS TO HIPPOCAMPUS AND FASCIA DENTATA OF RAT
CELLS OF ORIGIN OF ENTORHINAL CORTICAL AFFERENTS TO HIPPOCAMPUS AND FASCIA DENTATA OF RAT
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DOI：
10.1002/cne.901690306
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发表时间：
1976-01-01
影响因子：
2.5
通讯作者：
SCOVILLE, SA
中科院分区：
文献类型：
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作者：
STEWARD, O;SCOVILLE, SA
从内嗅皮质区到海马结构的通路由2个亚系统组成，其中一个亚系统主要投射到同侧海马固有的齿状筋膜和下区，另一个亚系统投射到双侧上级区。研究了这两种途径是否可能起源于内嗅区内的不同细胞群。这些内嗅通路的起源细胞通过辣根过氧化物酶（HRP）逆行标记来鉴定。注射标记的内嗅终末领域在两个筋膜齿和区域上级导致逆行标记的2个群体的细胞内嗅区。在注射的同侧，在第II层的细胞（主要是星状细胞）和第III层的细胞（主要是锥体细胞）中发现HRP反应产物。在注射液的近侧，反应产物几乎仅在第III层的细胞中发现。在上级区域选择性注射内嗅终末野，仅标记第III层的细胞，但这些细胞是双侧标记的。选择性注射的内嗅终末领域的筋膜齿导致在第二层的细胞的标记，但不是第三层，和这些细胞的第二层标记几乎完全同侧。在注射的对侧发现极少量的标记细胞在层II中。注射海马结构后，在前下托或旁下托中均未发现标记细胞。这些细胞群被发现能够逆行运输HRP，因为在前下托和旁下托的细胞被标记后，HRP注射到对侧内嗅区。向齿状筋膜的投射显然起源于第II层细胞，而向上级区的投射则起源于内嗅区固有的第III层细胞。从内嗅区到对侧齿状区的非常轻微的交叉投影可能来源于对侧齿状区HRP注射后标记的第II层中的小细胞群。
The pathway from the entorhinal cortical region to the hippocampal formation is comprised of 2 sub-systems, one of which projects predominantly to the ipsilateral fascia dentata and regio inferior of the hippocampus proper, and a second which projects bilaterally to regio superior. Whether these 2 pathways might originate from different cell populations within the entorhinal area was investigated. The cells of origin of these entorhinal pathways were identified by retrograde labeling with horseradish peroxidase (HRP). Injections which labeled the entorhinal terminal fields in both the fascia dentata and regio superior resulted in the retrograde labeling of 2 populations of cells in the entorhinal area. Ipsilateral to the injection, HRP reaction product was found in the cells of layer II (predominantly stellate cells) and the cells of layer III (predominantly pyramidal cells). Contralateral to the injections the reaction product was found almost exclusively in the cells of layer III. With selective injections of the entorhinal terminal field in regio superior, only the cells of layer III were labeled, but these were labeled bilaterally. Selective injection of the entorhinal terminal field in the fascia dentata resulted in the labeling of cells of layer II, but not of layer III, and these cells of layer II were labeled almost exclusively ipsilaterally. A very small number of labeled cells in layer II were found contralateral to the injection. No labeled cells were found either in the presubiculum or parasubiculum following injections of the hippocampal formation. These cell populations were found capable of retrograde transport of HRP since cells in both presubiculum and parasubiculum were labeled following HRP injections into the contralateral entorhinal area. The projections to the fascia dentata apparently originate from the cells of layer II, while the projections to regio superior originate from the cells of layer III of the entorhinal region proper. The very slight crossed projection from the entorhinal area to the contralateral area dentata probably originates from the small population of cells in layer II which are labeled following HRP injections in the contralateral area dentata.