Immunological identification of the heart myoglobin radical formed by hydrogen peroxide
Immunological identification of the heart myoglobin radical formed by hydrogen peroxide
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DOI：
10.1016/s0891-5849(02)00895-x
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发表时间：
2002-08-01
影响因子：
7.4
通讯作者：
Mason, RP
中科院分区：
文献类型：
--
作者：
Detweiler, CD;Deterding, LJ;Mason, RP
本研究报告的蛋白质自由基的检测使用特定的自由基反应的硝酮自旋陷阱结合硝酮抗体特异性。开发了结合硝酮自旋捕获物5，5-二甲基-1-吡咯啉N-氧化物（DMPO）的蛋白质加合物的多克隆抗体。抗体用于检测DMPO蛋白加合物产生的马肌红蛋白自过氧化。蛋白质印迹分析表明，肌红蛋白形成的主要自由基衍生的硝酮加合物在大鼠心脏上清液。出版社：Elsevier Science Inc.
This study reports the detection of protein free radicals using the specific free radical reactivity of nitrone spin traps in conjunction with nitrone-antibody specificity. Polyclonal antibodies were developed that bind to protein adducts of the nitrone spin-trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO). The antibodies were used to detect DMPO protein adducts produced on horse myoglobin resulting from self-peroxidation. Western blot analysis demonstrates that myoglobin forms the predominant radical-derived nitrone adduct in rat heart Supernatant. Published by Elsevier Science Inc.