INCREASES IN THE NUMBER OF REACTIVE GLIA IN THE VISUAL-CORTEX OF MACACA-FASCICULARIS FOLLOWING SUBCLINICAL LONG-TERM METHYL MERCURY EXPOSURE
INCREASES IN THE NUMBER OF REACTIVE GLIA IN THE VISUAL-CORTEX OF MACACA-FASCICULARIS FOLLOWING SUBCLINICAL LONG-TERM METHYL MERCURY EXPOSURE
复制标题
DOI：
10.1006/taap.1994.1244
复制
发表时间：
1994-12-01
影响因子：
3.8
通讯作者：
BURBACHER, TM
中科院分区：
文献类型：
--
作者：
CHARLESTON, JS;BOLENDER, RP;BURBACHER, TM
利用光学体积分体技术估计了长期亚临床暴露于甲基汞（MeHg）和氯化汞（无机汞；IHg）后成年雌性束状猕猴calcarine沟皮层的神经元、星形胶质细胞、反应性胶质细胞、少突胶质细胞、内皮细胞和周细胞的数量。四组猴子分别在6、12、18、12个月的时间里，通过口腔接触甲基汞（50 μ g Hg/kg体重/天），然后在6个月的时间里不接触甲基汞（清除组）。第五组猴子通过留置导管等速静脉输注IHg (HgCl2, 200 μ g Hg/kg body wt/day)，持续3个月。反应性胶质细胞的数量在每个治疗组都有显著增加，6个月增加72%，12个月增加152%，18个月MeHg暴露组增加120%，清除组的反应性胶质细胞数量仍然升高（89%）。IHg暴露组反应性胶质细胞数量增加了165%。IHg暴露组和清除组组织中甲基汞含量较低；然而，两组的IHg水平都有所升高。这些结果表明IHg可能是反应性胶质细胞增加的原因。所有其他类型的细胞，包括神经元，在规定的暴露水平和持续时间内，数量没有显着变化。在亚临床长期接触汞后，神经胶质细胞群的变化对反应性神经胶质细胞的特性和神经元的长期功能和存活能力的影响进行了讨论。(C) 1994学术出版社，Inc.
The number of neurons, astrocytes, reactive glia, oligodendrocytes, endothelia, and pericytes in the cortex of the calcarine sulcus of adult female Macaca fascicularis following long-term subclinical exposure to methyl mercury (MeHg) and mercuric chloride (inorganic mercury; IHg) has been estimated by use of the optical volume fractionator stereology technique. Four groups of monkeys were exposed to MeHg (50 mu g Hg/kg body wt/day) by mouth for 6, 12, 18, and 12 months followed by 6 months without exposure (clearance group). A fifth group of monkeys was administered IHg (as HgCl2; 200 mu g Hg/kg body wt/day) by constant rate intravenous infusion via an indwelling catheter for 3 months. Reactive glia showed a significant increase in number for every treatment group, increasing 72% in the 6-month, 152% in the 12-month, and 120% in the 18-month MeHg exposed groups, and the number of reactive glia in the clearance group remained elevated (89%). The IHg exposed group showed a 165% increase in the number of reactive glia. The IHg exposed group and the clearance group had low levels of MeHg present within the tissue; however, the level of IHg was elevated in both groups. These results suggest that the IHg may be responsible for the increase in reactive glia. All other cell types, including the neurons, showed no significant change in number at the prescribed exposure level and durations. The identities of the reactive glial cells and the implications for the long-term function and survivability of the neurons due to changes in the glial population following subclinical long-term exposure to mercury are discussed. (C) 1994 Academic Press, Inc.