Skeletal muscle adaptations to microgravity exposure in the mouse
Skeletal muscle adaptations to microgravity exposure in the mouse
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DOI：
10.1152/japplphysiol.00603.2003
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发表时间：
2003-12-01
影响因子：
3.3
通讯作者：
Leinwand, LA
中科院分区：
文献类型：
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作者：
Harrison, BC;Allen, DL;Leinwand, LA
为了研究微重力对小鼠骨骼肌纤维大小、肌肉收缩蛋白和酶活性的影响，将日龄雌性C57BL/6J小鼠分为动物围栏模块(AEM)地面对照组和航天(SF)处理组。SF动物乘坐奋进号航天飞机(STS-108/UF-1)飞行，并接受类似的11天和19小时的微重力。肌纤维横截面积的免疫组织化学分析显示，在所分析的每一块肌肉中，与AEM对照相比，SF的所有纤维类型的平均肌纤维横截面积显著减少(P<；0.0001)。在比目鱼肌中，肌球蛋白重链(MHC)亚型表达的免疫组织化学分析显示，表达MHC IIx和MHC ILB的肌纤维百分比显著增加(P<；0.05)。在腓肠肌和足底肌中，MHC亚型的表达没有明显变化。对于被分析的肌肉，没有观察到MHC I或MHC IIa蛋白表达的变化。对腓肠肌的酶分析显示，与AEM对照组相比，SF组的柠檬酸合成酶活性显著降低。
To investigate the effects of microgravity on murine skeletal muscle fiber size, muscle contractile protein, and enzymatic activity, female C57BL/6J mice, aged 64 days, were divided into animal enclosure module (AEM) ground control and spaceflight (SF) treatment groups. SF animals were flown on the space shuttle Endeavour (STS-108/UF-1) and subjected to similar to11 days and 19 h of microgravity. Immunohistochemical analysis of muscle fiber cross-sectional area revealed that, in each of the muscles analyzed, mean muscle fiber cross-sectional area was significantly reduced (P < 0.0001) for all fiber types for SF vs. AEM control. In the soleus, immunohistochemical analysis of myosin heavy chain (MHC) isoform expression revealed a significant increase in the percentage of muscle fibers expressing MHC IIx and MHC Ilb (P < 0.05). For the gastrocnemius and plantaris, no significant changes in MHC isoform expression were observed. For the muscles analyzed, no alterations in MHC I or MHC IIa protein expression were observed. Enzymatic analysis of the gastrocnemius revealed a significant decrease in citrate synthase activity in SF vs. AEM control.