Expression and function of periostin-isoforms in bone
Expression and function of periostin-isoforms in bone
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DOI：
10.1002/jcb.20115
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发表时间：
2004-08-01
影响因子：
4
通讯作者：
Safadi, FF
中科院分区：
文献类型：
--
作者：
Litvin, J;Selim, AH;Safadi, FF
骨膜蛋白最初在MC 3 T3-E1成骨细胞样细胞中鉴定。我们已经确定了骨膜蛋白的亚型称为骨膜蛋白样因子（PLF）。它与其他蛋白质如成束蛋白I（fasl）、MPB 70、betaIG-H3和Algal-CAM同源。所有这些蛋白质都参与调节细胞粘附。PLF和骨膜蛋白的其他同种型在它们的C-末端序列上不同。PLF和骨膜蛋白在两个特定区域不同，在673和699氨基酸（aa）之间和785-812 aa之间。骨膜蛋白同种型在成骨细胞分化和成熟阶段在体内和体外表达。它们的mRNA存在于前成骨细胞中，如通过原位杂交检测到的，并且蛋白质的大小在86和93 kD之间，如通过Western印迹分析确定的。反义寡核苷酸和抗体针对骨膜蛋白的亚型被用来阻止这些蛋白质的活性。在这两种情况下，成骨细胞特异性分化标志物的水平显着降低，表明这些蛋白质在成骨细胞分化中的作用。(C)2004 Wiley-Liss，Inc.
Periostin was originally identified in MC3T3-E1 osteoblast-like cells. We have identified an isoform of periostin referred to as periostin-like-factor (PLF). It is homologous to other proteins such as fasciclin I (fas 1), MPB70, betaIG-H3, and Algal-CAMs. All of these proteins are implicated in regulating cell adhesion. PLF and the other isoforms of periostin differ in their C-terminal sequences. PLF and periostin differ in two specific regions, between 673 and 699 amino acids (aa) and 785-812 aa. Periostin isoforms are expressed in vivo and in vitro during the stages of osteoblast differentiation and maturation. Their mRNAs are present in pre-osteoblast cells as detected by in situ hybridization, and the proteins are between 86 and 93 kD in size as determined by Western blot analysis. Antisense oligonucleotides and antibodies directed against the isoforms of periostin were used to block the activity of these proteins. In both cases, the levels of osteoblast-specific-differentiation markers were markedly reduced suggesting a role for these proteins in osteoblast differentiation. (C) 2004 Wiley-Liss, Inc.