Identification of Staphylococcus aureus and determination of methicillin resistance directly from positive blood cultures by isothermal amplification and a disposable detection device
Identification of Staphylococcus aureus and determination of methicillin resistance directly from positive blood cultures by isothermal amplification and a disposable detection device
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DOI：
10.1128/jcm.02234-07
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发表时间：
2008-04-01
影响因子：
9.4
通讯作者：
Tang, Yi-Wei
中科院分区：
文献类型：
--
作者：
Goldmeyer, James;Li, Haijing;Tang, Yi-Wei
开发了一种简单、快速和用户友好的方法来鉴定金黄色葡萄球菌，并直接从含有聚集性的血液培养基中革兰阳性球菌确定其对甲氧西林的耐药性。在用等温解旋酶依赖的扩增方法扩增nuc和mecA基因之前，样品被稀释和加热。使用一次性检测设备检测扩增产物。检测金黄色葡萄球菌和甲氧西林耐药性的临床敏感性和特异性分别为100%和98%。
A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively.