Immunophenotypic analysis of feline haemolymphatic neoplasia using flow cytometry
Immunophenotypic analysis of feline haemolymphatic neoplasia using flow cytometry
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使用流式细胞术对猫血淋巴肿瘤进行免疫表型分析
DOI：
10.1007/bf00638924
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
E. Hoover
中科院分区：
文献类型：
--
作者：
G. Dean;P. Groshek;N. Jain;E. Hoover
采用表型特异性单克隆抗体（MAbs）和流式细胞术对17只疑似血淋巴瘤形成的猫的组织进行了分析。8例病例还根据法国-美国-英国（FAB）急性白血病系统进行了分类，并通过细胞化学染色进行了表征。单克隆抗体组鉴定的表型包括T淋巴细胞、CD 4+淋巴细胞、CD 8+淋巴细胞、未分化淋巴细胞、红系或髓系。将细胞学诊断与表型相结合，17例中有15例获得准确诊断。在15例成功分型的病例中，8例为T细胞型，2例为B细胞型，1例为髓系型，2例为红系型，2例为红系和髓系混合型。在尸检时，确定不与单克隆抗体组反应的两份样本为非血淋巴源性。在7只FeLV阳性猫中的3只中证明了肿瘤细胞表达猫白血病病毒（FeLV）。缺乏B淋巴细胞特异性单克隆抗体是抗体组中最显著的缺陷，并排除了B细胞白血病的结论性鉴定。FAB分型为鉴别骨髓增生异常综合征和骨髓增生性疾病提供了一种更客观的方法。细胞化学染色证实了非淋巴瘤的流式细胞术结果，但在其他方面没有完善的表型诊断的流式细胞术分析。在这里介绍的情况下，免疫表型与流式细胞术和我们的小组单克隆抗体，准确地确定淋巴与非淋巴瘤，以及单一和混合谱系骨髓增生性疾病。
Tissues from 17 cats with suspected haemolymphatic neoplasia were analysed using phenotypespecific monoclonal antibodies (MAbs) and flow cytometry. Eight of the cases were also classified according to the French-American-British (FAB) system for acute leukaemias and were characterised by cytochemical staining. Phenotypes identified by the panel of MAb employed here included T lymphocyte, CD4+ lymphocyte, CD8+ lymphocyte, undifferentiated lymphocyte, erythroid or myeloid. By combining the cytological diagnosis of neoplasia with the phenotype, a precise diagnosis was obtained in 15 of the 17 cases. Of the 15 cases successfully phenotyped, eight were T-cell, two were B-cell, one was myeloid, two were erythroid, and two were mixed erythroid and myeloid. Two samples that did not react with the panel of MAb were determined at necropsy to be of non-haemolymphatic origin. Expression of feline leukaemia virus (FeLV) by neoplastic cells was demonstrated in three of seven FeLV-positive cats. The lack of a B-lymphocyte-specific MAb was the most significant deficit in the antibody panel and precluded conclusive identification of B-cell leukaemias. The FAB classification provided a more objective means to identify myelodysplastic syndrome versus myeloproliferative disorder. Cytochemical staining confirmed flow cytometric results in non-lymphoid neoplasias but otherwise did not refine the phenotypic diagnosis made by flow cytometric analysis. In the cases presented here, immunophenotyping with flow cytometry and our panel of MAbs, accurately identified lymphoid versus non-lymphoid neoplasia, as well as single and mixed lineage myeloproliferative disorders.
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免疫表型可预测 T 系急性淋巴细胞白血病和 T 系非霍奇金淋巴瘤的放射抗性。
DOI：
10.1016/0360-3016(92)90718-w
发表时间：
1992
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
Uckun,FM;Song,CW;Nesbit,M;Kersey,JH;Ramsay,NK
通讯作者：
Ramsay,NK
由单克隆抗体 (FT2) 定义的猫胸腺细胞抗原可识别具有特定细胞毒性的非辅助细胞亚群。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Klotz,FW;Cooper,MD
通讯作者：
Cooper,MD
影响因子：
20.3
作者：
James;-D.;Griffin;Roger;Davis;Douglas;-A.;Nelson;Frederick;-R.;Davey;Robert;-J.;Mayer;Charles;Schiffer;Ross;Mcintyre;Clara;Bloomfield
通讯作者：
James;-D.;Griffin;Roger;Davis;Douglas;-A.;Nelson;Frederick;-R.;Davey;Robert;-J.;Mayer;Charles;Schiffer;Ross;Mcintyre;Clara;Bloomfield
猫淋巴瘤：免疫学和细胞化学特征。
影响因子：
11.2
作者：
Rojko,JL;Kociba,GJ;Abkowitz,JL;Hamilton,KL;HardyJr,WD;Ihle,JN;O'Brien,SJ
通讯作者：
O'Brien,SJ
与 CD5 样分子反应的猫 T 细胞特异性单克隆抗体的表征。
影响因子：
1
作者：
Ackley,CD;Cooper,MD
通讯作者：
Cooper,MD