Listeria monocytogenes induces mast cell extracellular traps
Listeria monocytogenes induces mast cell extracellular traps
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DOI：
10.1016/j.imbio.2016.08.006
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发表时间：
2017-02-01
期刊：
影响因子：
2.8
通讯作者：
Chacon-Salinas, Rommel
中科院分区：
文献类型：
--
作者：
Campillo-Navarro, Marcia;Leyva-Paredes, Kahiry;Chacon-Salinas, Rommel
肥大细胞在包括变态反应和感染性疾病在内的不同免疫学现象中发挥重要作用。几种细菌诱导肥大细胞活化，导致脱粒和几种细胞因子和趋化因子的产生。然而，肥大细胞也具有不同的杀微生物活性，如吞噬作用和释放DNA。嵌入的颗粒蛋白质称为肥大细胞胞外陷阱（MCETs）。虽然以前的报告表明，细胞外细菌能够诱导MCETs很少知道，如果细胞内细菌可以诱导这些结构。在这项工作中，我们评估了MCETs诱导细胞内细菌单核细胞增生李斯特菌。我们发现肥大细胞在L.单核细胞增多症，这种DNA与组蛋白和类胰蛋白酶复合。在胞外DNA释放前，L.单核细胞增生诱导肥大细胞核膜的修饰，在细胞核外检测到DNA。L.单核细胞增生刺激肥大细胞产生显著量的活性氧（ROS），阻断NADPH氧化酶减少肥大细胞的DNA释放。最后，MCETs对单核细胞增生李斯特菌显示出抗微生物活性，当β-氨基己糖苷酶活性被抑制时，该活性被部分阻断。这些结果表明，单核细胞增多性李斯特菌诱导肥大细胞产生杀微生物的MCET，表明肥大细胞在抑制感染中的作用超出了炎症的诱导。(C)2016年爱思唯尔有限公司。All rights reserved.
Mast cells play an essential role in different immunological phenomena including allergy and infectious diseases. Several bacteria induce mast cell activation leading to degranulation and the production of several cytokines and chemokines. However, mast cells also have different microbicidal activities such as phagocytosis and the release of DNA with. embedded granular proteins known as Mast Cell Extracellular Traps (MCETs). Although previous reports indicate that extracellular bacteria are able to induce MCETs little is known if intracellular bacteria can induce these structures. In this work, we evaluated MCETs induction by the intracellular bacteria Listeria monocytogenes. We found that mast cells released DNA after stimulation with L. monocytogenes, and this DNA was complexed to histone and tryptase. Before extracellular DNA release, L. monocytogenes induced modifications to the mast cell nuclear envelope and DNA was detected outside the nucleus. L. monocytogenes stimulated mast cells to produce significant amounts of reactive oxygen species (ROS) and blocking NADPH oxidase diminished DNA release by mast cells. Finally, MCETs showed antimicrobial activity against L monocytogenes that was partially blocked when beta-hexosaminidase activity was inhibited. These results show that L monocytogenes induces mast cells to produce microbicidal MCETs, suggesting a role for mast cells in containing infection beyond the induction of inflammation. (C) 2016 Elsevier GmbH. All rights reserved.